D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signalling.
D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signalling.
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独脚金内酯（SLs）是一类新的类胡萝卜素衍生的植物激素，对植物的发育过程、植物结构的形成以及与寄生杂草和共生的灌木菌根真菌的相互作用至关重要。尽管对SL生物合成途径的研究进展迅速，但对SL的感知和信号传导机制仍知之甚少。在这里，我们发现DWARF53 （D53）作为SL信号的抑制因子，SL诱导其降解。我们发现，与野生型植株相比，水稻d53突变体产生的分蘖数量夸张，这是由功能获得突变引起的，对外源SL处理不敏感。D53基因产物与I类Clp atp酶蛋白具有相同的预测特征，并且可以与α/β水解酶蛋白DWARF14 （D14）和F-box蛋白DWARF3 （D3）形成复合物，这两种先前确定的信号成分可能负责SL感知。我们证明，在D14和d3依赖的方式下，SLs诱导蛋白酶体降解D53，并消除其促进腋芽生长的活性。我们的遗传和生化数据表明，D53作为SL信号通路的抑制因子，其激素诱导的降解是SL感知和反应之间的关键分子联系。
Strigolactones (SLs) are a new class of carotenoid-derived phytohormones essential for developmental processes shaping plant architecture and interactions with parasitic weeds and symbiotic arbuscular mycorrhizal fungi. Despite the rapid progress in elucidating the SL biosynthetic pathway, the perception and signaling mechanisms of SL remain poorly understood. Here we show that DWARF53 (D53) acts as a repressor of SL signaling and SLs induce its degradation. We found that the rice d53 mutant, which produces an exaggerated number of tillers compared to wild type plants, is caused by a gain-of-function mutation and is insensitive to exogenous SL treatment. The D53 gene product shares predicted features with the class I Clp ATPase proteins and can form a complex with the α/β hydrolase protein DWARF14 (D14) and the F-box protein DWARF3 (D3), two previously identified signaling components potentially responsible for SL perception. We demonstrate that, in a D14- and D3-dependent manner, SLs induce D53 degradation by the proteasome and abrogate its activity in promoting axillary bud outgrowth. Our combined genetic and biochemical data reveal that D53 acts as a repressor of the SL signaling pathway, whose hormone-induced degradation represents a key molecular link between SL perception and responses.
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