Macrophage Fate Mapping.
Macrophage Fate Mapping.
复制标题
DOI：
10.1002/cpz1.456
复制
发表时间：
2022-06
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
组织驻留巨噬细胞存在于所有组织中，在这些组织中它们执行内稳态和免疫监视功能。在许多组织中，驻留巨噬细胞由胚胎祖细胞发育而来，这些祖细胞通过局部增殖成熟为一个自我维持的群体。然而，在组织生长、感染或无菌性炎症等情况下，组织驻留巨噬细胞可得到招募的单核细胞衍生巨噬细胞的支持。循环血液单核细胞来源于造血干细胞祖细胞，并具有独特的基因谱，这些基因谱支持其在组织内的其他功能。确定组织内的细胞起源（个体发生）和细胞更新对于理解单核细胞和巨噬细胞对组织内稳态和疾病的贡献变得非常重要。命运图谱绘制，或谱系追踪，是一种基于驱动报告系统的独特基因表达来追踪细胞的有前景的方法，通常是在Cre - 重组酶介导的切除事件的下游表达一种荧光蛋白。这种方法通常根据发育阶段、疾病发作时间或与炎症消退的关键阶段相关联来适时应用。重要的是，髓系命运图谱绘制可与许多新兴技术相结合，包括单细胞RNA测序和空间成像。髓系细胞命运图谱绘制方法的应用使得在疾病模型中关于髓系个体发生、组织驻留以及单核细胞命运方面有了重大发现。本实验方案概述将讨论多种髓系命运图谱绘制方法，包括在成年和胚胎组织中的组成型和诱导型标记方法。本文概述了在小鼠中用于巨噬细胞命运图谱绘制的基本方法和模型。© 2022作者。由威利期刊有限责任公司出版的《现代实验技术》。
基本方案1：成年命运图谱绘制
基本方案2：胚胎命运图谱绘制
Tissue‐resident macrophages are present in all tissues where they perform homeostatic and immune surveillance functions. In many tissues, resident macrophages develop from embryonic progenitors, which mature into a self‐maintaining population through local proliferation. However, tissue‐resident macrophages can be supported by recruited monocyte‐derived macrophages during scenarios such as tissue growth, infection, or sterile inflammation. Circulating blood monocytes arise from hematopoietic stem cell progenitors and possess unique gene profiles that support additional functions within the tissue. Determining cell origins (ontogeny) and cellular turnover within tissues has become important to understanding monocyte and macrophage contributions to tissue homeostasis and disease. Fate mapping, or lineage tracing, is a promising approach to tracking cells based on unique gene expression driving reporter systems, often downstream of a Cre‐recombinase–mediated excision event, to express a fluorescent protein. This approach is typically deployed temporally with developmental stage, disease onset, or in association with key stages of inflammation resolution. Importantly, myeloid fate mapping can be combined with many emerging technologies, including single‐cell RNA‐sequencing and spatial imaging. The application of myeloid cell fate mapping approaches has allowed for impactful discoveries regarding myeloid ontogeny, tissue residency, and monocyte fate within disease models. This protocol outline will discuss a variety of myeloid fate mapping approaches, including constitutive and inducible labeling approaches in adult and embryo tissues. This article outlines basic approaches and models used in mice for fate mapping macrophages. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Adult Fate Mapping Basic Protocol 2: Embryonic Fate Mapping
登录
查看更多内容
影响因子：
4.6
作者：
Hameyer, Dorothe;Loonstra, Ate;Bockamp, Ernesto
通讯作者：
Bockamp, Ernesto
影响因子：
32.4
作者：
Croxford, Andrew L.;Lanzinger, Margit;Becher, Burkhard
通讯作者：
Becher, Burkhard
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
3
作者：
Clausen, BE;Burkhardt, C;Förster, I
通讯作者：
Förster, I
影响因子：
6.9
作者：
Golde, WT;Gollobin, P;Rodriguez, LL
通讯作者：
Rodriguez, LL