Fe-S cluster biogenesis in Gram-positive bacteria: SufU is a zinc-dependent sulfur transfer protein.
Fe-S cluster biogenesis in Gram-positive bacteria: SufU is a zinc-dependent sulfur transfer protein.
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DOI：
10.1021/bi4011978
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发表时间：
2014-01-14
期刊：
影响因子：
2.9
通讯作者：
Dos Santos, Patricia C.
中科院分区：
文献类型：
--
作者：
Selbach, Bruna P.;Chung, Alexander H.;Scott, Aubrey D.;George, Simon J.;Cramer, Stephen P.;Dos Santos, Patricia C.
The biosynthesis of Fe-S clusters in Bacillus subtilis and other Gram-positive bacteria is catalyzed by the SufCDSUB system. The first step in this pathway involves the sulfur mobilization from the free amino acid cysteine to a sulfur acceptor protein SufU via a PLP-dependent cysteine desulfurase SufS. In this reaction scheme, the formation of an enzyme S-covalent intermediate is followed by the binding of SufU. This event leads to the second half of the reaction where a deprotonated thiol of SufU promotes the nucleophilic attack onto the persulfide intermediate of SufS. Kinetic analysis combined with spectroscopic methods identified that the presence of a zinc atom tightly bound to SufU (Ka=1017 M−1) is crucial for its structural and catalytic competency. Fe-S cluster assembly experiments showed that despite the high degree of sequence and structural similarity to the ortholog enzyme IscU, the B. subtilis SufU does not act as a standard Fe-S cluster scaffold protein. The involvement of SufU as a dedicated agent of sulfur transfer, rather than as an assembly scaffold, in the biogenesis of Fe-S clusters in Gram-positive microbes indicates distinct strategies used by bacterial systems to assemble Fe-S clusters. Description. SufU has high affinity for zinc. Inactivation of SufU upon incubation with increasing concentration of TPEN was quantified through its sulfurtransferase activity in SufS assays (◆) and the amount of zinc-bound the protein (●). The calculated Ka is 1017. The background shows the active site of the B. subtilis SufU protein (PDB 2AZH) highlighting the conserved residues, Cys41, Cys66, and Cys128, and Asp43 coordinating the zinc atom.
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