Spinal Cord Injury Suppresses Cutaneous Inflammation: Implications for Peripheral Wound Healing.
Spinal Cord Injury Suppresses Cutaneous Inflammation: Implications for Peripheral Wound Healing.
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脊髓损伤抑制皮肤炎症：对周围伤口愈合的影响。
DOI：
10.1089/neu.2016.4611
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发表时间：
2017
影响因子：
4.2
通讯作者：
Popovich,PhillipG
中科院分区：
文献类型：
--
作者：
Marbourg,JessicaM;Bratasz,Anna;Mo,Xiaokui;Popovich,PhillipG
遭受创伤性脊髓损伤（SCI）的人患皮肤病并发症的风险增加。这些情况增加了护理费用、再住院的发生率和发生其他感染的风险。SCI后皮肤并发症的后果可能会因神经免疫信号的损伤后缺陷而进一步加剧。事实上，功能性免疫系统对于最佳宿主防御和组织修复至关重要。在这里，我们测试了这样一个假设，即脊髓高位SCI会引起全身免疫抑制，并会抑制损伤水平以下的皮肤炎症。C57 BL/6小鼠接受SCI（T3脊髓水平）或假损伤;然后一天后，在损伤水平下方皮下注射完全弗氏佐剂（CFA）。通过给小鼠注射V-Sense（一种选择性标记巨噬细胞的全氟化碳（PFC）示踪剂），然后进行体内成像来量化炎症。总辐射效率，这是成比例的巨噬细胞的数量，在4天的时间内在CFA注射部位进行测量。放射性核素活体成像显示，在整个分析期间，SCI小鼠的巨噬细胞反应与假损伤小鼠相比减少了约50%。使用磁共振成像（MRI）证实辐射效率数据，并且这些数据共同表明SCI显著损害皮下炎症。未来的研究应该确定增强局部炎症或增强全身免疫功能是否可以提高SCI患者皮肤伤口愈合的速度或效率。这样做还可以限制伤口感染或SCI后愈合受损的继发性并发症。
People who suffer a traumatic spinal cord injury (SCI) are at increased risk for developing dermatological complications. These conditions increase cost of care, incidence of rehospitalization, and the risk for developing other infections. The consequences of dermatological complications after SCI are likely exacerbated further by post-injury deficits in neural-immune signaling. Indeed, a functional immune system is essential for optimal host defense and tissue repair. Here, we tested the hypothesis that SCI at high spinal levels, which causes systemic immune suppression, would suppress cutaneous inflammation below the level of injury. C57BL/6 mice received an SCI (T3 spinal level) or sham injury; then one day later complete Freund's adjuvant (CFA) was injected subcutaneously below the injury level. Inflammation was quantified by injecting mice with V-Sense, a perfluorocarbon (PFC) tracer that selectively labels macrophages, followed byin vivoimaging. The total radiant efficiency, which is proportional to the number of macrophages, was measured over a 4-day period at the site of CFA injection. Fluorescentin vivoimaging revealed that throughout the analysis period, the macrophage reaction in SCI mice was reduced ∼50% compared with sham-injured mice. Radiant efficiency data were confirmed using magnetic resonance imaging (MRI), and together the data indicate that SCI significantly impairs subcutaneous inflammation. Future studies should determine whether enhancing local inflammation or boosting systemic immune function can improve the rate or efficiency of cutaneous wound healing in individuals with SCI. Doing so also could limit wound infections or secondary complications of impaired healing after SCI.
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影响因子：
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