Differential carbohydrate recognition by Campylobacter jejuni strain 11168: influences of temperature and growth conditions.
Differential carbohydrate recognition by Campylobacter jejuni strain 11168: influences of temperature and growth conditions.
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DOI：
10.1371/journal.pone.0004927
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Korolik V
中科院分区：
文献类型：
--
作者：
Day CJ;Tiralongo J;Hartnell RD;Logue CA;Wilson JC;von Itzstein M;Korolik V
致病性临床菌株NCTC 11168是第一个被测序的空肠弯曲杆菌菌株，并且已经被广泛用于研究C.空肠发病机制然而，C.已证明空肠NCTC 11168极大地影响其定殖潜力。对C. jejuni NCTC 11168，使用在各种条件下生长或维持的频繁传代的、非定殖的、基因组测序的（11168-GS）和不频繁传代的、原始的、毒性的（11168-O）分离株。被C识别和结合的聚糖结构。空肠中含有末端甘露糖、N-乙酰神经氨酸、半乳糖和岩藻糖。值得注意的是，发现只有在室温下用正常氧气挑战时，11168-O才始终与唾液酸或末端甘露糖结构结合，而11168-GS与这些结构结合，而与生长/维持条件无关。此外，未加帽的半乳糖和岩藻糖基化结构的结合显着减少时，C。空肠在大气氧条件下保持在25 ° C。通过聚糖阵列分析鉴定的这些结合差异通过特异性凝集素竞争性抑制C.空肠至Caco-2肠细胞系。我们的数据表明，甘露糖和/或N-乙酰神经氨酸的结合可能提供了初始的相互作用，重要的殖民环境暴露后。
The pathogenic clinical strain NCTC11168 was the first Campylobacter jejuni strain to be sequenced and has been a widely used laboratory model for studying C. jejuni pathogenesis. However, continuous passaging of C. jejuni NCTC11168 has been shown to dramatically affect its colonisation potential. Glycan array analysis was performed on C. jejuni NCTC11168 using the frequently passaged, non-colonising, genome sequenced (11168-GS) and the infrequently passaged, original, virulent (11168-O) isolates grown or maintained under various conditions. Glycan structures recognised and bound by C. jejuni included terminal mannose, N-acetylneuraminic acid, galactose and fucose. Significantly, it was found that only when challenged with normal oxygen at room temperature did 11168-O consistently bind to sialic acid or terminal mannose structures, while 11168-GS bound these structures regardless of growth/maintenance conditions. Further, binding of un-capped galactose and fucosylated structures was significantly reduced when C. jejuni was maintained at 25°C under atmospheric oxygen conditions. These binding differences identified through glycan array analysis were confirmed by the ability of specific lectins to competitively inhibit the adherence of C. jejuni to a Caco-2 intestinal cell line. Our data suggests that the binding of mannose and/or N-acetylneuraminic acid may provide the initial interactions important for colonisation following environmental exposure.
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