Antagonistic role of vitamin D3 and retinoic acid on the differentiation of chicken hematopoietic macrophages into osteoclast precursor cells.
Antagonistic role of vitamin D3 and retinoic acid on the differentiation of chicken hematopoietic macrophages into osteoclast precursor cells.
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维生素D3和视黄酸对鸡造血巨噬细胞向破骨细胞前体细胞分化的拮抗作用
DOI：
10.1210/endo.136.1.7828561
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发表时间：
1995
期刊：
影响因子：
4.8
通讯作者：
P. Jurdic
中科院分区：
文献类型：
--
作者：
C. Woods;C. Domenget;F. Solari;Olivier Gandrillon;E. Lazarides;P. Jurdic
本文描述了一种体外培养的破骨细胞分化模型，该模型来源于鸡卵黄囊和外周血巨噬细胞的均质群体。在原代培养中，两种类型的巨噬细胞都经历增殖期，在达到高细胞密度后进入静止状态，然后聚集并最终形成大的多核巨细胞（MNGCs），可能是通过融合形成的。根据几种形态学和生化标准，这些MNGCs可以被描述为过早的破骨细胞，尽管它们没有经历最终的分化步骤，使它们能够在体外吸收骨。单细胞衍生集落的克隆分析表明，在这些培养条件下，所有巨噬细胞都有可能分化成这些破骨细胞样细胞。维甲酸和1,25-二羟基维生素D3 [1,25-(OH)2D3]都能调节巨噬细胞的生长，但以拮抗的方式。虽然维甲酸强烈促进巨噬细胞增殖，阻碍MNGC形成，但1,25-(OH)2D3抑制巨噬细胞增殖，改变MNGC形成动力学。联合实验发现维甲酸诱导的增殖信号可以覆盖1,25-(OH)2D3诱导的分化信号。我们的研究结果表明，尽管维甲酸和维生素D3通过同源受体起作用，但它们对巨噬细胞向破骨细胞祖细胞分化的作用却截然相反。
An in vitro culture model of osteoclast differentiation is described which is derived from homogeneous populations of chick yolk sac and peripheral blood macrophages. In primary cultures, both types of macrophages undergo a proliferative phase, become quiescent after reaching high cell densities, then aggregate and eventually form large multinucleated giant cells (MNGCs), presumably by fusion. These MNGCs can be characterized as premature osteoclasts on the basis of several morphological and biochemical criteria, although they do not undergo the final differentiation step rendering them competent to resorb bone in vitro. Clonal analysis of single cell-derived colonies indicates that all macrophages have the potential to differentiate into these osteoclast-like cells under these culture conditions. Both retinoic acid and 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] modulate macrophage growth, but in an antagonistic manner. Although retinoic acid strongly promotes macrophage proliferation and impedes MNGC formation, 1,25-(OH)2D3 inhibits proliferation and changes the kinetics of MNGC formation. Combination experiments reveal that the proliferative signals induced by retinoic acid can override the signal to differentiate induced by 1,25-(OH)2D3. Our results indicate that even though retinoic acid and vitamin D3 act through homologous receptors, they have dramatically opposing effects on macrophage differentiation toward osteoclast progenitors.
DOI：
10.1073/pnas.87.12.4828
发表时间：
1990-06-01
影响因子：
11.1
作者：
WIKTORJEDRZEJCZAK, W;BARTOCCI, A;STANLEY, ER
通讯作者：
STANLEY, ER
影响因子：
4.8
作者：
KURIHARA, N;CHENU, C;ROODMAN, GD
通讯作者：
ROODMAN, GD
HL-60 细胞分化为具有破骨细胞表型的细胞。
影响因子：
4.8
作者：
Yoneda,T;Alsina,MM;Garcia,JL;Mundy,GR
通讯作者：
Mundy,GR