Identification and comparative profiling of miRNAs in an early flowering mutant of trifoliate orange and its wild type by genome-wide deep sequencing.
Identification and comparative profiling of miRNAs in an early flowering mutant of trifoliate orange and its wild type by genome-wide deep sequencing.
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通过全基因组深度测序鉴定和比较枇杷早花突变体及其野生型中的 miRNA
DOI：
10.1371/journal.pone.0043760
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang JZ
中科院分区：
文献类型：
--
作者：
Sun LM;Ai XY;Li WY;Guo WW;Deng XX;Hu CG;Zhang JZ
microRNAs（miRNAs）是一类新型的内源性小RNA，通过在转录后水平对基因表达产生负面影响，在多种生物和代谢过程中发挥调控作用。虽然已知的拟南芥和水稻miRNAs的数量不断增加，但有关木本植物如柑橘的miRNAs的信息仍然有限。对枳橙子的早花突变体（早熟枳橙子，Poncirus trifoliata L.）Raf.）结果在4个文库中共获得99个家族的141个已知miRNAs和75个新miRNAs。基于51个新的miRNAs家族，共预测了317个潜在的靶基因，GO和KEGG注释表明，高排名的miRNAs靶基因是那些参与植物发育、转录、蛋白质降解和交叉适应等多种细胞过程的基因。为了表征突变体及其野生型在幼年和成年发育阶段表达的那些miRNA，通过实时PCR对几种miRNA的表达谱进行进一步分析。结果显示，无论是突变体还是野生型，与幼年期相比，大多数miRNA在成年期均下调。这些结果表明，保守和新发现的miRNAs在柑橘生长发育、逆境响应等生理过程中发挥着重要作用。
MicroRNAs (miRNAs) are a new class of small, endogenous RNAs that play a regulatory role in various biological and metabolic processes by negatively affecting gene expression at the post-transcriptional level. While the number of known Arabidopsis and rice miRNAs is continuously increasing, information regarding miRNAs from woody plants such as citrus remains limited. Solexa sequencing was performed at different developmental stages on both an early flowering mutant of trifoliate orange (precocious trifoliate orange, Poncirus trifoliata L. Raf.) and its wild-type in this study, resulting in the obtainment of 141 known miRNAs belonging to 99 families and 75 novel miRNAs in four libraries. A total of 317 potential target genes were predicted based on the 51 novel miRNAs families, GO and KEGG annotation revealed that high ranked miRNA-target genes are those implicated in diverse cellular processes in plants, including development, transcription, protein degradation and cross adaptation. To characterize those miRNAs expressed at the juvenile and adult development stages of the mutant and its wild-type, further analysis on the expression profiles of several miRNAs through real-time PCR was performed. The results revealed that most miRNAs were down-regulated at adult stage compared with juvenile stage for both the mutant and its wild-type. These results indicate that both conserved and novel miRNAs may play important roles in citrus growth and development, stress responses and other physiological processes.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
影响因子：
7.2
作者：
Cardon, GH;Hohmann, S;Huijser, P
通讯作者：
Huijser, P
影响因子：
11.6
作者：
Aukerman, MJ;Sakai, H
通讯作者：
Sakai, H
血清中 microRNA 的表征：一类用于诊断癌症和其他疾病的新型生物标志物
影响因子：
44.1
作者：
Chen, Xi;Ba, Yi;Zhang, Chen-Yu
通讯作者：
Zhang, Chen-Yu