Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions.
Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions.
复制标题
绘制组织间相互作用的皮肤基底膜的分子和结构特化图。
DOI：
10.1038/s41467-021-22881-y
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发表时间：
2021-05-10
影响因子：
16.6
通讯作者：
Fujiwara H
中科院分区：
文献类型：
--
作者：
Tsutsui K;Machida H;Nakagawa A;Ahn K;Morita R;Sekiguchi K;Miner JH;Fujiwara H
组织间的相互作用是多细胞的基础。虽然基底膜（BM）位于组织界面，但其在组织间相互作用中的作用模式仍然知之甚少，主要是因为BM在不同组织间界面的分子和结构细节仍然不清楚。通过结合定量转录组学和免疫组化，我们系统地确定了细胞来源，分子身份和组织分布的细胞外基质分子在小鼠毛囊，并揭示BM的组成和架构是精致的专门为不同的组织间的相互作用，包括上皮细胞，上皮-肌肉和上皮-神经的相互作用。上皮-成纤维细胞界面，即毛胚-真皮乳头界面，在BM中产生不对称组织的侧特异性异质性，由新表征的界面、钩状和网状BM定义。这些BM的一种组分层粘连蛋白α5是毛发周期调节和毛胚-真皮乳头锚定所需的。我们的研究强调了BM异质性在不同组织间相互作用中的重要性。基底膜位于组织界面，但它如何介导不同的组织间相互作用尚不清楚。在这里，作者系统地定义了皮肤基底膜组成的空间异质性，并显示其在组织间相互作用中的功能重要性。
Inter-tissue interaction is fundamental to multicellularity. Although the basement membrane (BM) is located at tissue interfaces, its mode of action in inter-tissue interactions remains poorly understood, mainly because the molecular and structural details of the BM at distinct inter-tissue interfaces remain unclear. By combining quantitative transcriptomics and immunohistochemistry, we systematically identify the cellular origin, molecular identity and tissue distribution of extracellular matrix molecules in mouse hair follicles, and reveal that BM composition and architecture are exquisitely specialized for distinct inter-tissue interactions, including epithelial–fibroblast, epithelial–muscle and epithelial–nerve interactions. The epithelial–fibroblast interface, namely, hair germ–dermal papilla interface, makes asymmetrically organized side-specific heterogeneity in the BM, defined by the newly characterized interface, hook and mesh BMs. One component of these BMs, laminin α5, is required for hair cycle regulation and hair germ–dermal papilla anchoring. Our study highlights the significance of BM heterogeneity in distinct inter-tissue interactions. The basement membrane is located at tissue interfaces, but how it mediates distinct inter-tissue interactions is unclear. Here, the authors systematically define the spatial heterogeneity of skin basement membrane composition and show its functional importance in inter-tissue interactions.
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影响因子：
64.5
作者：
Fujiwara H;Ferreira M;Donati G;Marciano DK;Linton JM;Sato Y;Hartner A;Sekiguchi K;Reichardt LF;Watt FM
通讯作者：
Watt FM
影响因子：
10.5
作者：
de Lau W;Peng WC;Gros P;Clevers H
通讯作者：
Clevers H
影响因子：
23.9
作者：
Brownell, Isaac;Guevara, Elizabeth;Bai, C. Brian;Loomis, Cynthia A.;Joyner, Alexandra L.
通讯作者：
Joyner, Alexandra L.
影响因子：
4.6
作者：
Kendal, Adrian R.;Layton, Thomas;Carr, Andrew
通讯作者：
Carr, Andrew
影响因子：
16.6
作者：
Angelidis, Ilias;Simon, Lukas M.;Schiller, Herbert B.
通讯作者：
Schiller, Herbert B.