Integrated Proteotranscriptomics Reveals Differences in Molecular Immunity between Min and Large White Pig Breeds.
Integrated Proteotranscriptomics Reveals Differences in Molecular Immunity between Min and Large White Pig Breeds.
复制标题
整合蛋白质转录组学揭示小型和大型白猪品种之间分子免疫的差异
作者：
免疫系统在免疫防御、稳定、监视、耐受和调节中起着至关重要的作用。长期的选择或进化是控制猪抗病性发展的重要因素。为了更好地了解不同抗病水平的分子机制，我们利用转录组学和蛋白质组学分析了中外猪种之间的免疫差异，从而确定参与免疫调节的关键基因和蛋白质。参与这些途径的关键基因可以作为发现有用的多态性的候选基因，这些多态性可以包括在育种计划中，以选择天然抗性动物。本研究结果将为抗病育种提供新的见解。摘要长期的选择或进化是决定猪抗病性发展的重要因素。为了更好地阐明不同抗病水平的分子机制，我们使用转录组学和蛋白质组学分析来表征在相同环境中饲养的6头抗性猪（民猪）和6头易感猪（大白色，LW）之间的免疫差异。共鉴定出135个蛋白质和791个基因在大白色猪和民猪组之间差异表达。蛋白质表达聚类和功能分析表明，与免疫系统过程、体液免疫应答、B细胞受体信号通路、淋巴细胞介导的免疫和天然免疫应答相关的蛋白质在民猪中表达量较高。通过转录组基因集富集分析，发现细胞粘附分子和抗原加工呈递途径在民猪中显著富集。综合蛋白质组学和转录组学数据分析确定了16个在mRNA和蛋白质水平上差异表达的基因。此外，这16个基因中有13个与免疫疾病的数量性状位点相关，包括参与先天免疫的神经EGFL样基因2（NELL 2）和乳酸脱氢酶B（LDHB）。基因/蛋白质和细胞因子之间的相关性分析显示LW猪中上调的蛋白质与免疫抑制/促炎细胞因子如白细胞介素（IL）10、IL 6和肿瘤坏死因子α相关。使用平行反应监测分析进一步验证了这一点。综上所述，我们发现了几个潜在的候选途径和关键基因/蛋白参与决定两个研究的猪品种之间的抗病性差异，这可能会提供新的见解，猪抗病育种。
Simple Summary The immune system plays a vital role in immune defense, stability, surveillance, tolerance, and regulation. Long-term selection or evolution is an important factor governing the development of disease resistance in pigs. To better understand the molecular mechanisms underlying different levels of disease resistance, we used transcriptomics and proteomics analysis to analyze the immune differences between Chinese and foreign pig breeds and thereby identify critical genes and proteins involved in immune regulation. Key genes involved in these pathways could act as candidates for the discovery of useful polymorphisms that could be included in breeding programs to enable the selection of naturally resistant animals. The results of this study will provide new insights into breeding pigs for disease resistance. Abstract Long-term selection or evolution is an important factor governing the development of disease resistance in pigs. To better clarify the molecular mechanisms underlying different levels of disease resistance, we used transcriptomics and proteomics analysis to characterize differences in the immunities between six resistant (Min pig) and six susceptible (Large White, LW) pigs which were raised in the same environment. A total of 135 proteins and 791 genes were identified as being differentially expressed between the Large White and Min pig groups. Protein expression clustering and functional analysis revealed that proteins related to immune system process, humoral immune response, the B cell receptor signaling pathway, lymphocyte-mediated immunity, and innate immune responses were more highly expressed in Min pigs. Transcriptome gene set enrichment analysis was used to reveal that pathways of cell adhesion molecules and antigen processing and presentation are significantly enriched in Min pigs. Integrated proteomics and transcriptomics data analysis identified 16 genes that are differentially expressed at both the mRNA and protein levels. In addition, 13 out of these 16 genes were related to the quantitative trait loci of immune diseases, including neural EGFL-like 2 (NELL2) and lactate dehydrogenase B (LDHB), which are involved in innate immunity. Correlation analysis between the genes/proteins and cytokines shows upregulated proteins in LW pigs in association with immunosuppressive/pro-inflammatory cytokines, such as interleukin (IL) 10, IL6, and tumor necrosis factor alpha. This was further validated using parallel reaction monitoring analysis. In summary, we discovered several potential candidate pathways and key genes/proteins involved in determining differences in disease resistance between the two studied pig breeds, which could provide new insights into the breeding of pigs for disease resistance.
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影响因子：
3.6
作者：
Liu HH;Hu Y;Zheng M;Suhoski MM;Engleman EG;Dill DL;Hudnall M;Wang J;Spolski R;Leonard WJ;Peltz G
通讯作者：
Peltz G
DOI：
10.1186/cc8850
发表时间：
2010
期刊：
Critical care (London, England)
影响因子：
--
作者：
Anas A;van der Poll T;de Vos AF
通讯作者：
de Vos AF
影响因子：
64.8
作者：
Namkoong, Ho;Edahiro, Ryuya;Takano, Tomomi;Nishihara, Hiroshi;Shirai, Yuya;Sonehara, Kyuto;Tanaka, Hiromu;Azekawa, Shuhei;Mikami, Yohei;Lee, Ho;Hasegawa, Takanori;Okudela, Koji;Okuzaki, Daisuke;Motooka, Daisuke;Kanai, Masahiro;Naito, Tatsuhiko;Yamamoto, Kenichi;Wang, Qingbo S.;Saiki, Ryunosuke;Ishihara, Rino;Matsubara, Yuta;Hamamoto, Junko;Hayashi, Hiroyuki;Yoshimura, Yukihiro;Tachikawa, Natsuo;Yanagita, Emmy;Hyugaji, Takayoshi;Shimizu, Eigo;Katayama, Kotoe;Kato, Yasuhiro;Morita, Takayoshi;Takahashi, Kazuhisa;Harada, Norihiro;Naito, Toshio;Hiki, Makoto;Matsushita, Yasushi;Takagi, Haruhi;Aoki, Ryousuke;Nakamura, Ai;Harada, Sonoko;Sasano, Hitoshi;Kabata, Hiroki;Masaki, Katsunori;Kamata, Hirofumi;Ikemura, Shinnosuke;Chubachi, Shotaro;Okamori, Satoshi;Terai, Hideki;Morita, Atsuho;Asakura, Takanori;Sasaki, Junichi;Morisaki, Hiroshi;Uwamino, Yoshifumi;Nanki, Kosaku;Uchida, Sho;Uno, Shunsuke;Nishimura, Tomoyasu;Ishiguro, Takashi;Isono, Taisuke;Shibata, Shun;Matsui, Yuma;Hosoda, Chiaki;Takano, Kenji;Nishida, Takashi;Kobayashi, Yoichi;Takaku, Yotaro;Takayanagi, Noboru;Ueda, Soichiro;Tada, Ai;Miyawaki, Masayoshi;Yamamoto, Masaomi;Yoshida, Eriko;Hayashi, Reina;Nagasaka, Tomoki;Arai, Sawako;Kaneko, Yutaro;Sasaki, Kana;Tagaya, Etsuko;Kawana, Masatoshi;Arimura, Ken;Takahashi, Kunihiko;Anzai, Tatsuhiko;Ito, Satoshi;Endo, Akifumi;Uchimura, Yuji;Miyazaki, Yasunari;Honda, Takayuki;Tateishi, Tomoya;Tohda, Shuji;Ichimura, Naoya;Sonobe, Kazunari;Sassa, Chihiro Tani;Nakajima, Jun;Nakano, Yasushi;Nakajima, Yukiko;Anan, Ryusuke;Arai, Ryosuke;Kurihara, Yuko;Harada, Yuko;Nishio, Kazumi;Ueda, Tetsuya;Azuma, Masanori;Saito, Ryuichi;Sado, Toshikatsu;Miyazaki, Yoshimune;Sato, Ryuichi;Haruta, Yuki;Nagasaki, Tadao;Yasui, Yoshinori;Hasegawa, Yoshinori;Mutoh, Yoshikazu;Kimura, Tomoki;Sato, Tomonori;Takei, Reoto;Hagimoto, Satoshi;Noguchi, Yoichiro;Yamano, Yasuhiko;Sasano, Hajime;Ota, Sho;Nakamori, Yasushi;Yoshiya, Kazuhisa;Saito, Fukuki;Yoshihara, Tomoyuki;Wada, Daiki;Iwamura, Hiromu;Kanayama, Syuji;Maruyama, Shuhei;Yoshiyama, Takashi;Ohta, Ken;Kokuto, Hiroyuki;Ogata, Hideo;Tanaka, Yoshiaki;Arakawa, Kenichi;Shimoda, Masafumi;Osawa, Takeshi;Tateno, Hiroki;Hase, Isano;Yoshida, Shuichi;Suzuki, Shoji;Kawada, Miki;Horinouchi, Hirohisa;Saito, Fumitake;Mitamura, Keiko;Hagihara, Masao;Ochi, Junichi;Uchida, Tomoyuki;Baba, Rie;Arai, Daisuke;Ogura, Takayuki;Takahashi, Hidenori;Hagiwara, Shigehiro;Nagao, Genta;Konishi, Shunichiro;Nakachi, Ichiro;Murakami, Koji;Yamada, Mitsuhiro;Sugiura, Hisatoshi;Sano, Hirohito;Matsumoto, Shuichiro;Kimura, Nozomu;Ono, Yoshinao;Baba, Hiroaki;Suzuki, Yusuke;Nakayama, Sohei;Masuzawa, Keita;Namba, Shinichi;Suzuki, Ken;Naito, Yoko;Liu, Yu-Chen;Takuwa, Ayako;Sugihara, Fuminori;Wing, James B.;Sakakibara, Shuhei;Hizawa, Nobuyuki;Shiroyama, Takayuki;Miyawaki, Satoru;Kawamura, Yusuke;Nakayama, Akiyoshi;Matsuo, Hirotaka;Maeda, Yuichi;Nii, Takuro;Noda, Yoshimi;Niitsu, Takayuki;Adachi, Yuichi;Enomoto, Takatoshi;Amiya, Saori;Hara, Reina;Yamaguchi, Yuta;Murakami, Teruaki;Kuge, Tomoki;Matsumoto, Kinnosuke;Yamamoto, Yuji;Yamamoto, Makoto;Yoneda, Midori;Kishikawa, Toshihiro;Yamada, Shuhei;Kawabata, Shuhei;Kijima, Noriyuki;Takagaki, Masatoshi;Sasa, Noah;Ueno, Yuya;Suzuki, Motoyuki;Takemoto, Norihiko;Eguchi, Hirotaka;Fukusumi, Takahito;Imai, Takao;Fukushima, Munehisa;Kishima, Haruhiko;Inohara, Hidenori;Tomono, Kazunori;Kato, Kazuto;Takahashi, Meiko;Matsuda, Fumihiko;Hirata, Haruhiko;Takeda, Yoshito;Koh, Hidefumi;Manabe, Tadashi;Funatsu, Yohei;Ito, Fumimaro;Fukui, Takahiro;Shinozuka, Keisuke;Kohashi, Sumiko;Miyazaki, Masatoshi;Shoko, Tomohisa;Kojima, Mitsuaki;Adachi, Tomohiro;Ishikawa, Motonao;Takahashi, Kenichiro;Inoue, Takashi;Hirano, Toshiyuki;Kobayashi, Keigo;Takaoka, Hatsuyo;Watanabe, Kazuyoshi;Miyazawa, Naoki;Kimura, Yasuhiro;Sado, Reiko;Sugimoto, Hideyasu;Kamiya, Akane;Kuwahara, Naota;Fujiwara, Akiko;Matsunaga, Tomohiro;Sato, Yoko;Okada, Takenori;Hirai, Yoshihiro;Kawashima, Hidetoshi;Narita, Atsuya;Niwa, Kazuki;Sekikawa, Yoshiyuki;Nishi, Koichi;Nishitsuji, Masaru;Tani, Mayuko;Suzuki, Junya;Nakatsumi, Hiroki;Ogura, Takashi;Kitamura, Hideya;Hagiwara, Eri;Murohashi, Kota;Okabayashi, Hiroko;Mochimaru, Takao;Nukaga, Shigenari;Satomi, Ryosuke;Oyamada, Yoshitaka;Mori, Nobuaki;Baba, Tomoya;Fukui, Yasutaka;Odate, Mitsuru;Mashimo, Shuko;Makino, Yasushi;Yagi, Kazuma;Hashiguchi, Mizuha;Kagyo, Junko;Shiomi, Tetsuya;Fuke, Satoshi;Saito, Hiroshi;Tsuchida, Tomoya;Fujitani, Shigeki;Takita, Mumon;Morikawa, Daiki;Yoshida, Toru;Izumo, Takehiro;Inomata, Minoru;Kuse, Naoyuki;Awano, Nobuyasu;Tone, Mari;Ito, Akihiro;Nakamura, Yoshihiko;Hoshino, Kota;Maruyama, Junichi;Ishikura, Hiroyasu;Takata, Tohru;Odani, Toshio;Amishima, Masaru;Hattori, Takeshi;Shichinohe, Yasuo;Kagaya, Takashi;Kita, Toshiyuki;Ohta, Kazuhide;Sakagami, Satoru;Koshida, Kiyoshi;Hayashi, Kentaro;Shimizu, Tetsuo;Kozu, Yutaka;Hiranuma, Hisato;Gon, Yasuhiro;Izumi, Namiki;Nagata, Kaoru;Ueda, Ken;Taki, Reiko;Hanada, Satoko;Kawamura, Kodai;Ichikado, Kazuya;Nishiyama, Kenta;Muranaka, Hiroyuki;Nakamura, Kazunori;Hashimoto, Naozumi;Wakahara, Keiko;Sakamoto, Koji;Omote, Norihito;Ando, Akira;Kodama, Nobuhiro;Kaneyama, Yasunari;Maeda, Shunsuke;Kuraki, Takashige;Matsumoto, Takemasa;Yokote, Koutaro;Nakada, Taka-Aki;Abe, Ryuzo;Oshima, Taku;Shimada, Tadanaga;Harada, Masahiro;Takahashi, Takeshi;Ono, Hiroshi;Sakurai, Toshihiro;Shibusawa, Takayuki;Kimizuka, Yoshifumi;Kawana, Akihiko;Sano, Tomoya;Watanabe, Chie;Suematsu, Ryohei;Sageshima, Hisako;Yoshifuji, Ayumi;Ito, Kazuto;Takahashi, Saeko;Ishioka, Kota;Nakamura, Morio;Masuda, Makoto;Wakabayashi, Aya;Watanabe, Hiroki;Ueda, Suguru;Nishikawa, Masanori;Chihara, Yusuke;Takeuchi, Mayumi;Onoi, Keisuke;Shinozuka, Jun;Sueyoshi, Atsushi;Nagasaki, Yoji;Okamoto, Masaki;Ishihara, Sayoko;Shimo, Masatoshi;Tokunaga, Yoshihisa;Kusaka, Yu;Ohba, Takehiko;Isogai, Susumu;Ogawa, Aki;Inoue, Takuya;Fukuyama, Satoru;Eriguchi, Yoshihiro;Yonekawa, Akiko;Kan-o, Keiko;Matsumoto, Koichiro;Kanaoka, Kensuke;Ihara, Shoichi;Komuta, Kiyoshi;Inoue, Yoshiaki;Chiba, Shigeru;Yamagata, Kunihiro;Hiramatsu, Yuji;Kai, Hirayasu;Asano, Koichiro;Oguma, Tsuyoshi;Ito, Yoko;Hashimoto, Satoru;Yamasaki, Masaki;Kasamatsu, Yu;Komase, Yuko;Hida, Naoya;Tsuburai, Takahiro;Oyama, Baku;Takada, Minoru;Kanda, Hidenori;Kitagawa, Yuichiro;Fukuta, Tetsuya;Miyake, Takahito;Yoshida, Shozo;Ogura, Shinji;Abe, Shinji;Kono, Yuta;Togashi, Yuki;Takoi, Hiroyuki;Kikuchi, Ryota;Ogawa, Shinichi;Ogata, Tomouki;Ishihara, Shoichiro;Kanehiro, Arihiko;Ozaki, Shinji;Fuchimoto, Yasuko;Wada, Sae;Fujimoto, Nobukazu;Nishiyama, Kei;Terashima, Mariko;Beppu, Satoru;Yoshida, Kosuke;Narumoto, Osamu;Nagai, Hideaki;Ooshima, Nobuharu;Motegi, Mitsuru;Umeda, Akira;Miyagawa, Kazuya;Shimada, Hisato;Endo, Mayu;Ohira, Yoshiyuki;Watanabe, Masafumi;Inoue, Sumito;Igarashi, Akira;Sato, Masamichi;Sagara, Hironori;Tanaka, Akihiko;Ohta, Shin;Kimura, Tomoyuki;Shibata, Yoko;Tanino, Yoshinori;Nikaido, Takefumi;Minemura, Hiroyuki;Sato, Yuki;Yamada, Yuichiro;Hashino, Takuya;Shinoki, Masato;Iwagoe, Hajime;Takahashi, Hiroshi;Fujii, Kazuhiko;Kishi, Hiroto;Kanai, Masayuki;Imamura, Tomonori;Yamashita, Tatsuya;Yatomi, Masakiyo;Maeno, Toshitaka;Hayashi, Shinichi;Takahashi, Mai;Kuramochi, Mizuki;Kamimaki, Isamu;Tominaga, Yoshiteru;Ishii, Tomoo;Utsugi, Mitsuyoshi;Ono, Akihiro;Tanaka, Toru;Kashiwada, Takeru;Fujita, Kazue;Saito, Yoshinobu;Seike, Masahiro;Watanabe, Hiroko;Matsuse, Hiroto;Kodaka, Norio;Nakano, Chihiro;Oshio, Takeshi;Hirouchi, Takatomo;Makino, Shohei;Egi, Moritoki;Omae, Yosuke;Nannya, Yasuhito;Ueno, Takafumi;Katayama, Kazuhiko;Ai, Masumi;Fukui, Yoshinori;Kumanogoh, Atsushi;Sato, Toshiro;Hasegawa, Naoki;Tokunaga, Katsushi;Ishii, Makoto;Koike, Ryuji;Kitagawa, Yuko;Kimura, Akinori;Imoto, Seiya;Miyano, Satoru;Ogawa, Seishi;Kanai, Takanori;Fukunaga, Koichi;Okada, Yukinori
通讯作者：
Okada, Yukinori
影响因子：
5.8
作者：
Falck E;Karlsson S;Carlsson J;Helenius G;Karlsson M;Klinga-Levan K
通讯作者：
Klinga-Levan K
影响因子：
2.4
作者：
Mehrotra, A.;Bhushan, B.;Mishra, B. P.
通讯作者：
Mishra, B. P.