Noninvasive optical system for the study of the function of inner ear in living animals
Noninvasive optical system for the study of the function of inner ear in living animals
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用于研究活体动物内耳功能的无创光学系统
DOI：
10.1117/12.231687
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发表时间：
1996
影响因子：
2.5
通讯作者：
H. Rosskothen
中科院分区：
文献类型：
--
作者：
S. Khanna;C. Koester;J. Willemin;R. Daendliker;H. Rosskothen
听力器官具有卷曲结构，其中不同类型细胞的基本几何排列在整个器官中重复。不同哺乳动物的听力器官也使用相同的细胞排列。为了理解这种安排在处理听觉刺激方面的意义，测量活动物完整器官中的细胞功能是必不可少的。活着的动物完整内耳的感觉细胞可以通过圆窗膜在耳蜗底转处可视化。为了实现这一点，需要一个成像系统来检测来自内耳感觉细胞的微弱反射，同时拒绝来自感兴趣区域下面和上面的结构的强反射，特别是圆窗膜本身。开发和制造了一种共聚焦显微镜/干涉仪，以可视化感觉细胞并测量它们对施加到耳朵的声音的响应。介绍了该测量系统及其部分性能特点。
The Hearing Organ has a coiled structure in which the basic geometrical arrangement of the different types of cells is repeated throughout the organ. Hearing organs of different mammalian species also utilize the same arrangement of cells. In order to understand the significance of this arrangement in processing the auditory stimuli it is essential to measure the cellular function in an intact organ of a living animal. The sensory cells in the intact inner ear of a living animal can be visualized in the basal turn of the cochlea through the round window membrane. To achieve this an imaging system is needed to detect the weak reflections from the sensory cells in the inner ear while rejecting strong reflections from structures below and above the region of interest, particularly the round window membrane itself. A confocal microscope/interferometer was developed and built to visualize the sensory cells and to measure their vibration in response to sound applied to the ear. The measuring system and some of its performance characteristics are described.
DOI：
10.1073/pnas.91.4.1549
发表时间：
1994-02
影响因子：
11.1
作者：
S. V. van Netten;S. Khanna
通讯作者：
S. V. van Netten;S. Khanna