An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV.
An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV.
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嵌合表达 RBD 的灭活重组狂犬病病毒诱导针对 SARS-CoV-2 和 RABV 的体液和细胞免疫
DOI：
10.1016/j.virs.2022.12.006
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发表时间：
2023-04
影响因子：
5.5
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Zhang H;Jin H;Yan F;Song Y;Dai J;Jiao C;Bai Y;Sun J;Liu D;Wang S;Zhang M;Lu J;Huang J;Huang P;Li Y;Xia X;Wang H
许多研究表明，严重急性呼吸综合征冠状病毒2(SARS-CoV-2)可以感染多种动物，并在动物之间传播，甚至传播给人类，对人和动物构成威胁。迫切需要开发廉价高效的动物疫苗来预防和控制动物冠状病毒病2019(新冠肺炎)。狂犬病病毒(Radies Virus，RABV)是另一种重要的人畜共患病病原体，感染几乎所有温血动物，对公众健康构成极大威胁。本研究以RABV SRV9株的反向遗传系统为基础，构建了表达SARS-CoV-2武汉01株S1和RBD蛋白的重组嵌合病毒，并对其在小鼠、猫和狗体内的免疫原性进行了评价。结果表明，两种灭活的重组病毒均能诱导小鼠产生持久的抗SARS-CoV-2和RABV中和抗体，并产生较强的细胞免疫应答。值得注意的是，灭活的SRV-nCoV-RBD比SRV-nCoV-S1更早诱导抗体产生，后者在较长时间内保持高水平。灭活的SRV-nCoV-RBD在猫和狗体内诱导产生针对SARS-CoV-2和RABV的中和抗体，对SARS-CoV-2伪病毒包括α、β、伽玛、Delta和Omicron具有较广的交叉中和能力，有望成为动物用安全的新冠肺炎和狂犬病双价疫苗候选疫苗。灭活SRV-nCoV-RBD能诱导小鼠产生细胞免疫应答和产生CD4+CTM。灭活的SRV-nCoV-RBD在小鼠、猫和狗体内诱导产生抗SARS-CoV-2和RABV的中和抗体。SRV-nCoV-RBD诱导的中和抗体对SARS-CoV-2 VOCs具有广泛的交叉中和能力。
Many studies suggest that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect various animals and transmit among animals, and even to humans, posing a threat to humans and animals. There is an urgent need to develop inexpensive and efficient animal vaccines to prevent and control coronavirus disease 2019 (COVID-19) in animals. Rabies virus (RABV) is another important zoonotic pathogen that infects almost all warm-blooded animals and poses a great public health threat. The present study constructed two recombinant chimeric viruses expressing the S1 and RBD proteins of the SARS-CoV-2 Wuhan01 strain based on a reverse genetic system of the RABV SRV9 strain and evaluated their immunogenicity in mice, cats and dogs. The results showed that both inactivated recombinant viruses induced durable neutralizing antibodies against SARS-CoV-2 and RABV and a strong cellular immune response in mice. Notably, inactivated SRV-nCoV-RBD induced earlier antibody production than SRV-nCoV-S1, which was maintained at high levels for longer periods. Inactivated SRV-nCoV-RBD induced neutralizing antibodies against both SARS-CoV-2 and RABV in cats and dogs, with a relatively broad-spectrum cross-neutralization capability against the SARS-CoV-2 pseudoviruses including Alpha, Beta, Gamma, Delta, and Omicron, showing potential to be used as a safe bivalent vaccine candidate against COVID-19 and rabies in animals. Inactivated SRV-nCoV-RBD induces cellular immune response and the production of CD4+ CTM in mice. Inactivated SRV-nCoV-RBD induces neutralizing antibodies against SARS-CoV-2 and RABV in mice, cats and dogs. Neutralizing antibody induced by SRV-nCoV-RBD shows a broad cross-neutralization capability against SARS-CoV-2 VOCs.
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影响因子：
4.3
作者：
Jin, Hongli;Bai, Yujie;Wang, Hualei
通讯作者：
Wang, Hualei
影响因子：
13.8
作者：
Lassauniere, Ria;Polacek, Charlotta;Frische, Anders;Boding, Lasse;Saekmose, Susanne Gjorup;Rasmussen, Morten;Fomsgaard, Anders
通讯作者：
Fomsgaard, Anders
影响因子：
8.4
作者：
Liu, Juan;Liao, Min;Zhou, Jiyong
通讯作者：
Zhou, Jiyong
灭活狂犬病病毒载体中东呼吸综合征冠状病毒疫苗可诱导小鼠、骆驼和羊驼产生保护性免疫力
影响因子：
7.3
作者：
Chi H;Wang Y;Li E;Wang X;Wang H;Jin H;Han Q;Wang Z;Wang X;Zhu A;Sun J;Zhuang Z;Zhang L;Ye J;Wang H;Feng N;Hu M;Gao Y;Zhao J;Zhao Y;Yang S;Xia X
通讯作者：
Xia X
影响因子：
8.8
作者：
Hoffmann M;Zhang L;Krüger N;Graichen L;Kleine-Weber H;Hofmann-Winkler H;Kempf A;Nessler S;Riggert J;Winkler MS;Schulz S;Jäck HM;Pöhlmann S
通讯作者：
Pöhlmann S