Transepithelial electrical responses to Cl- of nonsensory region of gerbil utricle.
Transepithelial electrical responses to Cl- of nonsensory region of gerbil utricle.
复制标题
沙鼠椭圆囊非感觉区对 Cl- 的跨上皮电反应。
DOI：
10.1016/0005-2736(89)90454-9
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Marcus,NY
中科院分区：
文献类型：
--
作者：
Marcus,DC;Marcus,NY
沙鼠椭圆囊上皮片安装在一个micro-Ussing室，以确定和本地化的氯离子电导。在顶侧或基底侧灌注液中[Cl−]迅速降低（用羟乙基磺酸盐替代），并连续监测跨上皮电位差（Vt）和跨上皮电阻（Rt）。此外，还应用了已知抑制其他上皮细胞阴离子转运的药物。由于Cl−取代，Vt和Rt的所有初始变化方向与上皮两侧Cl−离子电导的存在一致。在存在和不存在基底外侧布美他尼的情况下，在心尖[Cl−]步骤期间Vt下降和Rt增加的时间过程和幅度在两种情况下是双相的并且相同。Vto基底外侧[Cl-]步的反应是双相的，布美他尼大大减弱了初始反应。这些发现表明，Cl−的最大电导位于基底外侧细胞膜，但细胞旁和/或顶端通路也具有有限的Cl−电导。3′，5-二氯二苯胺-2-羧酸（DCDPC）、5-硝基-2-（3-苯丙氨基）苯甲酸（NPPB）和4，4 ′-二异硫氰基二苯乙烯-2，2 ′-二磺酸（DIDS）均可引起Vt增加。NPPB和DIDS从根尖侧更有效。DCDPC和DIDS从心尖侧给药导致Rt降低。这些结果表明，这些药物在该组织中的作用是通过增强顶膜上的传导通路，而不是阻断基底外侧Cl−传导。
Sheets of utricular epithelium from gerbil were mounted in a micro-Ussing chamber in order to identify and localize chloride conductances. The [Cl−] was rapidly reduced (substituted with isethionate) in the apical or basolateral perfusate and the transepithelial potential difference (Vt) and transepithelial resistance (Rt) were monitored continuously. In addition, agents known to inhibit anion transport in other epithelia were applied. The direction of all initial changes inVtandRtdue to Cl−substitutions were consistent with the presence of ionic conductances for Cl−on both sides of the epithelium. The time-courses and magnitudes of the fall inVtand increase inRtduring apical [Cl−] steps in the presence and absence of basolateral bumetanide were monophasic and identical in the two cases. The response ofVtto basolateral [Cl−] steps was biphasic and the initial response was greatly attenuated by bumetanide. These findings demonstrate that the largest conductance for Cl−is in the basolateral cell membrane, but that the paracellular and/or apical pathway also possess a finite Cl−conductance. All three agents tested, 3′,5-dichlorodiphenylamine-2-carboxylic acid (DCDPC), 5-nitro-2(3-phenylpropylamino)benzoic acid (NPPB) and 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS), caused an increase inVt. NPPB and DIDS were more effective from the apical side. DCDPC and DIDS administered from the apical side led to a decrease inRt. These results suggest that these agents act in this tissue by enhancing a conductive pathway on the apical membrane rather than blocking the basolateral Cl−conductance.
阴离子两亲化合物抑制红细胞中的阴离子转运。
DOI：
--
发表时间：
1982
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
J. Cousin;R. Motais
通讯作者：
R. Motais
影响因子：
5.5
作者：
HODGKIN, AL;HOROWICZ, P
通讯作者：
HOROWICZ, P