Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes.
Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes.
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DOI：
10.1002/pmic.200800118
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发表时间：
2009-04
期刊：
影响因子：
3.4
通讯作者：
Goldberg, Zelanna
中科院分区：
文献类型：
--
作者：
Berglund, Susanne R.;Santana, Alison R.;Li, Dan;Rice, Robert H.;Rocke, David M.;Goldberg, Zelanna
人类接触砷和电离辐射的环境水平较低。虽然砷和电离辐射对人体健康的影响已分别进行了研究，但在剂量接近环境水平时，关于两者的综合影响的资料很少。砷的毒性可能受到同步电离辐射的影响，特别是考虑到它们在较高剂量下已知的个别致癌作用。我们发现角质形成细胞对低剂量砷和/或低剂量电离辐射暴露有反应，导致基于2DGE、免疫印迹和统计分析的差异蛋白质组学表达。7个蛋白通过严格的统计筛选在2DGE中进行差异表达，并通过严格的统计筛选进行后续免疫印迹。其中包括α-烯醇化酶、表皮脂肪酸结合蛋白、热休克蛋白27、组氨酸三核苷酸结合蛋白1、乳酸脱氢酶A、蛋白二硫异构酶前体和S100A9。四种蛋白质的综合效应不同于对任何一种毒物的反应。这些数据表明，对联合侮辱的可能反应与单独处理的反应有很大不同。有几种蛋白质的反应与高剂量暴露时不同，这增加了越来越多的文献，表明细胞对低剂量暴露的反应是不同的。
Human exposure to arsenic and ionizing radiation occur environmentally at low levels. While the human health effects of arsenic and ionizing radiation have been examined separately, there is little information regarding their combined effects at doses approaching environmental levels. Arsenic toxicity may be affected by concurrent ionizing radiation especially given their known individual carcinogenic actions at higher doses. We found that keratinocytes responded to either low dose arsenic and/or low dose ionizing radiation exposure, resulting in differential proteomic expression based on 2DGE, immunoblotting and statistical analysis. Seven proteins were identified that passed a rigorous statistical screen for differential expression in 2DGE and also passed a strict statistical screen for follow-up immunoblotting. These included: α-enolase, epidermal-fatty acid binding protein, heat shock protein 27, histidine triad nucleotide-binding protein 1, lactate dehydrogenase A, protein disulfide isomerase precursor and S100A9. Four proteins had combined effects that were different than would be expected based on the response to either individual toxicant. These data demonstrate a possible reaction to the combined insult that is substantially different from that of either separate treatment. Several proteins had different responses than what has been seen from high dose exposures, adding to the growing literature suggesting that the cellular responses to low dose exposures are distinct.
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影响因子：
3.4
作者：
Ding, LH;Shingyoji, M;Chen, DJ
通讯作者：
Chen, DJ
影响因子：
3.6
作者：
Moore, LE;Pfeiffer, R;Smith, AH
通讯作者：
Smith, AH
影响因子：
2.5
作者：
Katayama, Masafumi;Nakano, Hiroshi;Otsubo, Takehito
通讯作者：
Otsubo, Takehito
影响因子：
7
作者：
Chen, GA;Gharib, TG;Beer, DG
通讯作者：
Beer, DG
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y