Hydraulic Pressure during Fluid Flow Regulates Purinergic Signaling and Cytoskeleton Organization of Osteoblasts.
Hydraulic Pressure during Fluid Flow Regulates Purinergic Signaling and Cytoskeleton Organization of Osteoblasts.
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流体流动期间的液压调节果骨能信号传导和成骨细胞的细胞骨架组织。
DOI：
10.1007/s12195-014-0329-8
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发表时间：
2014-06-01
影响因子：
2.8
通讯作者：
Duncan, Randall L.
中科院分区：
文献类型：
--
作者：
Gardinier, Joseph D.;Gangadharan, Vimal;Wang, Liyun;Duncan, Randall L.
关键词：
在生理活动期间，成骨细胞经历各种机械力，这些机械力刺激新骨形成所必需的细胞水平的合成代谢反应。以前的研究主要是研究成骨细胞对各种形式的机械刺激的反应。然而，在这项研究中，我们评估了成骨细胞的反应，两个同时，但独立控制的刺激;流体流动诱导的剪切应力（FSS）和静态或循环静水压力（SHP或CHP，分别）。将MC 3 T3-E1成骨细胞样细胞与不同大小的SHP或CHP一起进行沿着12达因/cm 2的FSS，以确定压力是否增强成骨细胞在FSS期间的合成代谢反应。对于SHP和CHP，在FSS期间诱导ATP最大释放的液压的大小为15 mmHg。与FSS期间的15 mmHg相比，在FSS期间将液压增加到50 mmHg或100 mmHg会减弱ATP释放。在FSS到大气压的过程中，降低压力的大小减少ATP释放到静态细胞的基础ATP释放，并抑制肌动蛋白重组成应力纤维，这通常发生在FSS与15毫米汞柱的压力。相反，核因子κ B（NFκB）向细胞核的转运与液压的大小无关，并且被发现是通过磷脂酶C（PLC）的激活介导的，而不是src激酶。总之，发现FSS期间的液压以双相方式调节成骨细胞中的嘌呤能信号传导和肌动蛋白细胞骨架重组，而单独的FSS似乎刺激NFκB易位。了解液压对成骨细胞合成代谢反应的影响，在FSS可能提供急需的洞察力耦合机械刺激对成骨的生理效应。
During physiological activities, osteoblasts experience a variety of mechanical forces that stimulate anabolic responses at the cellular level necessary for the formation of new bone. Previous studies have primarily investigated the osteoblastic response to individual forms of mechanical stimuli. However in this study, we evaluated the response of osteoblasts to two simultaneous, but independently controlled stimuli; fluid flow-induced shear stress (FSS) and static or cyclic hydrostatic pressure (SHP or CHP, respectively). MC3T3-E1 osteoblasts-like cells were subjected to 12dyn/cm2 FSS along with SHP or CHP of varying magnitudes to determine if pressure enhances the anabolic response of osteoblasts during FSS. For both SHP and CHP, the magnitude of hydraulic pressure that induced the greatest release of ATP during FSS was 15 mmHg. Increasing the hydraulic pressure to 50 mmHg or 100 mmHg during FSS attenuated the ATP release compared to 15 mmHg during FSS. Decreasing the magnitude of pressure during FSS to atmospheric pressure reduced ATP release to that of basal ATP release from static cells and inhibited actin reorganization into stress fibers that normally occurred during FSS with 15 mmHg of pressure. In contrast, translocation of nuclear factor kappa B (NFκB) to the nucleus was independent of the magnitude of hydraulic pressure and was found to be mediated through the activation of phospholipase-C (PLC), but not src kinase. In conclusion, hydraulic pressure during FSS was found to regulate purinergic signaling and actin cytoskeleton reorganization in the osteoblasts in a biphasic manner, while FSS alone appeared to stimulate NFκB translocation. Understanding the effects of hydraulic pressure on the anabolic responses of osteoblasts during FSS may provide much needed insights into the physiologic effects of coupled mechanical stimuli on osteogenesis.
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DOI：
10.1083/jcb.153.3.491
发表时间：
2001-04-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Erb L;Liu J;Ockerhausen J;Kong Q;Garrad RC;Griffin K;Neal C;Krugh B;Santiago-Pérez LI;González FA;Gresham HD;Turner JT;Weisman GA
通讯作者：
Weisman GA
影响因子：
4.1
作者：
Chen, NX;Geist, DJ;Duncan, RL
通讯作者：
Duncan, RL
DOI：
10.1098/rsta.2009.0099
发表时间：
2009-09-13
影响因子：
5
作者：
Cowin, Stephen C.;Gailani, Gaffar;Benalla, Mohammed
通讯作者：
Benalla, Mohammed
影响因子：
3.4
作者：
Charras, GT;Horton, MA
通讯作者：
Horton, MA
影响因子：
2.4
作者：
Donahue, TLH;Haut, TR;Jacobs, CR
通讯作者：
Jacobs, CR