DNA methylation and regulation of the CD8A after duck hepatitis virus type 1 infection.
DNA methylation and regulation of the CD8A after duck hepatitis virus type 1 infection.
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DOI：
10.1371/journal.pone.0088023
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chen GH
中科院分区：
文献类型：
--
作者：
Xu Q;Chen Y;Zhao WM;Huang ZY;Zhang Y;Li X;Tong YY;Chang GB;Duan XJ;Chen GH
Cluster of differentiation 8 (CD8) is expressed in cytotoxic T cells, where it functions as a co-receptor for the T-cell receptor by binding to major histocompatibility complex class I (MHCI) proteins, which present peptides on the cell surface. CD8A is critical for cell-mediated immune defense and T-cell development. CD8A transcription is controlled by several cis-acting elements and trans-acting elements and is also regulated by DNA methylation. However, the epigenetic regulation of CD8A in the duck and its relationship with virus infection are still unclear. We investigated the epigenetic transcriptional regulatory mechanisms, such as DNA methylation, for the expression of the CD8A and further evaluated the contribution of such epigenetic regulatory mechanisms to DHV-I infection in the duck. Real-time quantitative polymerase chain reaction (RT-qPCR) revealed the highest level of CD8A expression to be in the thymus, followed by the lungs, spleen, and liver, and the levels of CD8A expression were very low in the kidney, cerebrum, cerebellum, and muscle in the duck. RT-qPCR also demonstrated that the CD8A mRNA was down-regulated significantly in morbid ducklings treated with DHV-1 and up-regulated significantly in non-morbid ducklings in all the tissues tested. In addition, hypermethylation of CD8A was detected in the morbid ducklings, whereas relatively low methylation of CD8A was evident in the non-morbid ducklings. The CD8A mRNA level was negatively associated with the CpG methylation level of CD8A and global methylation status. We concluded that the mRNA level of the CD8A was negatively associated with the CpG methylation level of CD8A and global methylation status in the duck, suggesting that the hypermethylation of CD8A may be associated with DHV-1 infection. The first two CpG sites of the CD8A promoter region could be considered as epigenetic biomarkers for resistance breeding against duckling hepatitis disease in the duck.
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影响因子：
4.5
作者：
Fernàndez-Roig S;Lai SC;Murphy MM;Fernandez-Ballart J;Quadros EV
通讯作者：
Quadros EV
影响因子：
56.9
作者：
Pestano, GA;Zhou, YL;Cantor, H
通讯作者：
Cantor, H
影响因子：
13.5
作者：
Wei, Y;Van Nhieu, JT;Buendia, MA
通讯作者：
Buendia, MA
PLK4杂合小鼠中polo样激酶CpG岛的异常甲基化。
影响因子：
3.8
作者：
Ward A;Morettin A;Shum D;Hudson JW
通讯作者：
Hudson JW
DOI：
10.1084/jem.178.3.941
发表时间：
1993-09-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Landry DB;Engel JD;Sen R
通讯作者：
Sen R