An immunoglobulin promoter displays cell-type specificity independently of the enhancer
An immunoglobulin promoter displays cell-type specificity independently of the enhancer
复制标题
免疫球蛋白启动子表现出独立于增强子的细胞类型特异性
作者：
John Foster;J. Stafford;C. Queen
最近将克隆的免疫球蛋白基因导入培养细胞的研究产生了两个重要的发现。首先，基因在导入淋巴细胞后表达，但非淋巴细胞1-3不表达。其次，免疫球蛋白基因的转录除了启动子区域外，还需要位于转录起始点4-10下游的增强子元件。这些发现提出了一个问题，即是启动子还是增强子区域对观察到的细胞类型特异性负责。事实上，已经证明免疫球蛋白增强剂只在淋巴细胞6、8、9起作用。我们在这里展示了免疫球蛋白κ轻链基因的启动子也对淋巴细胞具有很强的特异性。我们的结果再次强调了启动子相对于增强子在决定哪些细胞表达哪些基因方面的重要性。
Recent studies in which cloned immunoglobulin genes were introduced into cultured cells have produced two significant findings. First, the genes are expressed after transfection into lymphoid cells but not non-lymphoid cells1–3. Second, transcription of an immunoglobulin gene requires, in addition to the promoter region, an enhancer element located downstream of the transcription start site4–10. These findings raise the question of whether it is the promoter or the enhancer region that is responsible for the observed cell-type specificity. It has, in fact, been shown that immunoglobulin enhancers function only in lymphoid cells6,8,9. We show here that the promoter for an immunoglobulin κ light-chain gene also is strongly specific for lymphoid cells. Our result re-emphasizes the importance of promoters relative to enhancers in determining which cells express which genes.
转染淋巴细胞的免疫球蛋白重链基因的表达。
DOI：
10.1073/pnas.81.5.1292
发表时间：
1984
影响因子：
11.1
作者：
Deans,RJ;Denis,KA;Taylor,A;Wall,R
通讯作者：
Wall,R
DOI：
10.1073/pnas.81.9.2650
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
PARSLOW, TG;BLAIR, DL;GRANNER, DK
通讯作者：
GRANNER, DK
影响因子：
14.9
作者：
LOPATA, MA;CLEVELAND, DW;SOLLNERWEBB, B
通讯作者：
SOLLNERWEBB, B