E-cadherin expression in ovarian cancer in the laying hen, Gallus domesticus, compared to human ovarian cancer.
E-cadherin expression in ovarian cancer in the laying hen, Gallus domesticus, compared to human ovarian cancer.
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DOI：
10.1016/j.ygyno.2009.02.011
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发表时间：
2009-06
影响因子：
4.7
通讯作者：
Hales, Dale Buchanan
中科院分区：
文献类型：
--
作者：
Ansenberger, Kristine;Zhuge, Yan;Lagman, Jo Ann J.;Richards, Cassandra;Barua, Animesh;Bahr, Janice M.;Hales, Dale Buchanan
上皮性卵巢癌（EOC）是女性癌症死亡的主要原因。直到最近，缺乏适当的动物模型阻碍了卵巢癌早期检测标志物的发现。衰老的母鸡可以作为动物模型，因为它会自发地发生卵巢腺癌，其组织学外观与人类疾病相似。e -钙粘蛋白是一种粘附蛋白，在许多癌症中下调，但在原发性人类卵巢癌中被证明是上调的。我们的目的是评估e -钙粘蛋白在母鸡卵巢中的表达，并将其与人类卵巢癌的表达进行比较。选取185周龄的白来亨鸡（癌变和正常）作为样本。使用人卵巢肿瘤组织阵列与人类疾病进行比较。采用免疫组织化学（IHC）、免疫印迹（Western blot）和实时荧光定量PCR （qRT-PCR）分析了E-cadherin mRNA和蛋白在癌变和正常母鸡卵巢中的表达。用中性缓冲福尔马林固定组织进行免疫组化。Western blot分析液氮冷冻组织中的蛋白质。从RNAlater保存的组织中提取RNA，进行qRT-PCR分析。采用人卵巢肿瘤组织阵列进行免疫组化。qRT-PCR和Western blot结果显示，癌母鸡卵巢中E-cadherin mRNA和蛋白的表达量显著高于正常母鸡卵巢。免疫组化法观察到E-cadherin在人和母鸡卵巢癌组织中的表达相似。在原发卵巢肿瘤和癌母鸡的腹膜转移组织中也观察到类似的E-cadherin表达。我们的研究结果表明，E-cadherin的上调是卵巢癌的早期决定性事件，可能在原发性卵巢肿瘤的初始发展中发挥重要作用。e -钙粘蛋白在腹膜腔内继发性肿瘤的发展中似乎也很重要。我们的数据提示E-cadherin可能是预防治疗转移性卵巢癌的重要靶点，进一步证实了蛋鸡是研究人上皮性卵巢癌的良好模型。
Epithelial ovarian carcinoma (EOC) is a leading cause of cancer deaths in women. Until recently, a significant lack of an appropriate animal model has hindered the discovery of early detection markers for ovarian cancer. The aging hen serves as an animal model because it spontaneously develops ovarian adenocarcinomas similar in histological appearance to the human disease. E-cadherin is an adherens protein that is down-regulated in many cancers, but has been shown to be up-regulated in primary human ovarian cancer. Our objective was to evaluate E-cadherin expression in the hen ovary and compare its expression to human ovarian cancer. White Leghorn hens aged 185 weeks (cancerous and normal) were used for sample collection. A human ovarian tumor tissue array was used for comparison to the human disease. E-cadherin mRNA and protein expression were analyzed in cancerous and normal hen ovaries by immunohistochemistry (IHC), Western blot, and quantitative real-time PCR (qRT-PCR). Tissue fixed in neutral buffered formalin was used for IHC. Protein from tissue frozen in liquid nitrogen was analyzed by Western blot. RNA was extracted from tissue preserved in RNAlater and analyzed by qRT-PCR. The human ovarian tumor tissue array was used for IHC. E-cadherin mRNA and protein expression were significantly increased in cancerous hen ovaries as compared to ovaries of normal hens by qRT-PCR and Western blot. Similar expression of E-cadherin was observed by IHC in both human and hen ovarian cancer tissues. Similar E-cadherin expression was also observed in primary ovarian tumor and peritoneal metastatic tissue from cancerous hens. Our findings suggest that the up-regulation of E-cadherin is an early defining event in ovarian cancer and may play a significant role in the initial development of the primary ovarian tumor. E-cadherin also appears to be important in the development of secondary tumors within the peritoneal cavity. Our data suggest that E-cadherin may prove to be an important target in the preventative treatment of metastatic ovarian cancer and further confirm that the laying hen is a good model for the study of human epithelial ovarian carcinoma.
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影响因子：
50.5
作者：
Faleiro-Rodrigues, C;Macedo-Pinto, I;Lopes, CS
通讯作者：
Lopes, CS
影响因子：
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LAEMMLI, UK
通讯作者：
LAEMMLI, UK