Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins.
Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins.
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DOI：
10.1186/1471-2180-11-65
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发表时间：
2011-04-01
期刊：
影响因子：
4.2
通讯作者：
Musser JM
中科院分区：
文献类型：
--
作者：
Sitkiewicz I;Green NM;Guo N;Mereghetti L;Musser JM
血清型M28 A组链球菌（GAS）菌株MGAS 6180的基因组含有一个新的遗传元件，命名为差异区2（RD2），编码7个推定的分泌性胞外蛋白。RD2存在于所有血清型M28菌株和与女性泌尿生殖道感染相关的几种其他GAS血清型菌株中。我们在这里表明，气体RD2元件是存在于MGAS 6180菌株作为一个综合性的染色体形式和一个圆形的染色体外元素。RD2样区域在代表引起人类疾病的五种主要B群链球菌血清型中的三种的菌株的公开可获得的基因组序列中被鉴定。RD2编码的10个蛋白质与参与嗜热链球菌整合染色体元件（ICE）接合转移的蛋白质具有显著的相似性。我们通过过滤交配将RD2从GAS菌株MGAS 6180（血清型M28）转移到血清型M1和M4 GAS菌株。用丝裂霉素C（一种DNA损伤剂）处理MGAS 6180菌株后，RD2元件的拷贝数迅速显著增加。使用基于PCR的方法，我们还确定了RD2样区域在多组C和G菌株的停乳链球菌亚种。类马链球菌培养的侵袭性人类感染。总之，这些数据表明，RD2元件已传播的横向基因转移到遗传多样性菌株的人类致病性链球菌。
The genome of serotype M28 group A Streptococcus (GAS) strain MGAS6180 contains a novel genetic element named Region of Difference 2 (RD2) that encodes seven putative secreted extracellular proteins. RD2 is present in all serotype M28 strains and strains of several other GAS serotypes associated with female urogenital infections. We show here that the GAS RD2 element is present in strain MGAS6180 both as an integrative chromosomal form and a circular extrachromosomal element. RD2-like regions were identified in publicly available genome sequences of strains representing three of the five major group B streptococcal serotypes causing human disease. Ten RD2-encoded proteins have significant similarity to proteins involved in conjugative transfer of Streptococcus thermophilus integrative chromosomal elements (ICEs). We transferred RD2 from GAS strain MGAS6180 (serotype M28) to serotype M1 and M4 GAS strains by filter mating. The copy number of the RD2 element was rapidly and significantly increased following treatment of strain MGAS6180 with mitomycin C, a DNA damaging agent. Using a PCR-based method, we also identified RD2-like regions in multiple group C and G strains of Streptococcus dysgalactiae subsp.equisimilis cultured from invasive human infections. Taken together, the data indicate that the RD2 element has disseminated by lateral gene transfer to genetically diverse strains of human-pathogenic streptococci.
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影响因子：
64.8
作者：
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