Contribution of exogenous genetic elements to the group A Streptococcus metagenome.
Contribution of exogenous genetic elements to the group A Streptococcus metagenome.
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DOI：
10.1371/journal.pone.0000800
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发表时间：
2007-08-29
期刊：
影响因子：
3.7
通讯作者：
Musser JM
中科院分区：
文献类型：
--
作者：
Beres SB;Musser JM
细菌物种的菌株之间的基因含量的变化有助于表型（例如毒力和抗微生物剂抗性）的生物医学相关差异。A组链球菌（GAS）引起多种人类感染和后遗症，并表现出复杂的致病行为。为了加强我们对这种重要病原体的基因型-表型关系的理解，我们确定了表达M蛋白血清型（M2，M4和2 M12）的四种GAS菌株的完整基因组序列，这些血清型通常引起非侵入性和侵入性感染。这些序列进行了比较，与8个先前确定的GAS基因组和区域的基因内容进行了评估。与先前确定的基因组一致，每个新基因组的大小为1.9Mb，每个基因组的基因内容的10%编码在非编码的外源遗传元件上。与其他GAS基因组一样，这四个基因组是多溶原性的，并且前噬菌体编码每个基因组的大部分非溶原性基因内容。与大多数已确定的基因组不同，这些新的基因组中存在多个具有接合转座子和质粒特征的外源整合接合元件（ICE）。累积起来，鉴定出242个新的GAS宏基因组基因，这些基因不存在于先前测序的基因组中。重要的是，ICE占这四个基因组中鉴定的新GAS宏基因组基因含量的41%。两个大的ICE，命名为2096-RD.2（63 kb）和10750-RD.2（49 kb），具有多个编码抗微生物剂抗性的基因，分别包括四环素和红霉素。还驻留在这些ICE是三个基因编码推断的细胞外蛋白的未知功能，包括预测的细胞表面蛋白，仅存在于从急性链球菌感染后肾小球肾炎患者培养的血清型M12菌株的基因组中。这些数据提供了关于GAS宏基因组的新信息，并将有助于致病机制，抗菌素耐药性和群体基因组学的研究。
Variation in gene content among strains of a bacterial species contributes to biomedically relevant differences in phenotypes such as virulence and antimicrobial resistance. Group A Streptococcus (GAS) causes a diverse array of human infections and sequelae, and exhibits a complex pathogenic behavior. To enhance our understanding of genotype-phenotype relationships in this important pathogen, we determined the complete genome sequences of four GAS strains expressing M protein serotypes (M2, M4, and 2 M12) that commonly cause noninvasive and invasive infections. These sequences were compared with eight previously determined GAS genomes and regions of variably present gene content were assessed. Consistent with the previously determined genomes, each of the new genomes is ∼1.9 Mb in size, with ∼10% of the gene content of each encoded on variably present exogenous genetic elements. Like the other GAS genomes, these four genomes are polylysogenic and prophage encode the majority of the variably present gene content of each. In contrast to most of the previously determined genomes, multiple exogenous integrated conjugative elements (ICEs) with characteristics of conjugative transposons and plasmids are present in these new genomes. Cumulatively, 242 new GAS metagenome genes were identified that were not present in the previously sequenced genomes. Importantly, ICEs accounted for 41% of the new GAS metagenome gene content identified in these four genomes. Two large ICEs, designated 2096-RD.2 (63 kb) and 10750-RD.2 (49 kb), have multiple genes encoding resistance to antimicrobial agents, including tetracycline and erythromycin, respectively. Also resident on these ICEs are three genes encoding inferred extracellular proteins of unknown function, including a predicted cell surface protein that is only present in the genome of the serotype M12 strain cultured from a patient with acute poststreptococcal glomerulonephritis. The data provide new information about the GAS metagenome and will assist studies of pathogenesis, antimicrobial resistance, and population genomics.
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影响因子：
6.4
作者：
Banks, DJ;Porcella, SF;Musser, JM
通讯作者：
Musser, JM
影响因子：
4.9
作者：
Bingen, E;Leclercq, R;Cohen, R
通讯作者：
Cohen, R
影响因子：
5.2
作者：
Brenciani, A;Ojo, KK;Giovanetti, E
通讯作者：
Giovanetti, E
影响因子：
14.9
作者：
Chenna, R;Sugawara, H;Thompson, JD
通讯作者：
Thompson, JD
DOI：
10.1073/pnas.152298499
发表时间：
2002-07-23
影响因子：
11.1
作者：
Beres, SB;Sylva, GL;Musser, JM
通讯作者：
Musser, JM