Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro.
Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro.
复制标题
DOI：
10.3390/bioengineering8110165
复制
发表时间：
2021-10-29
期刊：
影响因子：
--
通讯作者：
Goodman SB
中科院分区：
文献类型：
--
作者：
Tsubosaka M;Maruyama M;Huang EE;Zhang N;Utsunomiya T;Gao Q;Shen H;Li X;Kushioka J;Hirata H;Yao Z;Yang YP;Goodman SB
转基因(GM)间充质干细胞(MSCs)和预适应MSCs(PMSCs)的使用可能为改善髓芯减压(CD)治疗早期股骨头坏死(ONFH)的疗效提供进一步的机会。本实验采用GM-IL-4高表达的MSCs(IL4-MSCs)、血小板衍生生长因子(PDGF-BB)高表达的MSCs(PDGF-BB-MSCs)和IL4-PDGF-BB共高表达的MSCs(IL4-PDGF-BB-MSCs)及其各自的PMSCs进行体外细胞增殖和成骨分化的比较。IL4-MSCs、PDGF-BB-MSCs、IL4-PDGF-BB-MSCs及各PMSC处理组较单纯MSC组显著促进细胞增殖。IL4-MSC组和IL4-PMSC组茜素红染色面积百分比明显低于MSC组。而PDGF-BB-MSC组茜素红染色面积百分比明显高于MSC组和PDGF-BB-PMSC组。IL4-PDGF-BB-PMSC组茜素红染色面积百分比明显高于IL4-PDGF-BB-MSC组。MSC组和IL4-PDGF-BB-PMSC组之间茜素红染色面积百分比差异无统计学意义。使用PDGF-BB-MSCs或IL4-PDGF-BB-PMSCs可促进细胞增殖。此外，PDGF-BB-MSCs促进成骨分化。GM-MSCs的加入可能为CD治疗ONFH提供一种有用的辅助细胞疗法。
The use of genetically modified (GM) mesenchymal stromal cells (MSCs) and preconditioned MSCs (pMSCs) may provide further opportunities to improve the outcome of core decompression (CD) for the treatment of early-stage osteonecrosis of the femoral head (ONFH). GM interleukin-4 (IL4) over-expressing MSCs (IL4-MSCs), platelet-derived growth factor (PDGF)-BB over-expressing MSCs (PDGF-BB-MSCs), and IL4-PDGF-BB co-over-expressing MSCs (IL4-PDGF-BB-MSCs) and their respective pMSCs were used in this in vitro study and compared with respect to cell proliferation and osteogenic differentiation. IL4-MSCs, PDGF-BB-MSCs, IL4-PDGF-BB-MSCs, and each pMSC treatment significantly increased cell proliferation compared to the MSC group alone. The percentage of Alizarin red-stained area in the IL4-MSC and IL4-pMSC groups was significantly lower than in the MSC group. However, the percentage of Alizarin red-stained area in the PDGF-BB-MSC group was significantly higher than in the MSC and PDGF-BB-pMSC groups. The percentage of Alizarin red-stained area in the IL4-PDGF-BB-pMSC was significantly higher than in the IL4-PDGF-BB-MSC group. There were no significant differences in the percentage of Alizarin red-stained area between the MSC and IL4-PDGF-BB-pMSC groups. The use of PDGF-BB-MSCs or IL4-PDGF-BB-pMSCs increased cell proliferation. Furthermore, PDGF-BB-MSCs promoted osteogenic differentiation. The addition of GM MSCs may provide a useful supplementary cell-based therapy to CD for treatment of ONFH.
登录
查看更多内容
影响因子：
7.5
作者：
Lin T;Pajarinen J;Nabeshima A;Lu L;Nathan K;Jämsen E;Yao Z;Goodman SB
通讯作者：
Goodman SB
影响因子：
3.7
作者：
Croes M;Oner FC;Kruyt MC;Blokhuis TJ;Bastian O;Dhert WJ;Alblas J
通讯作者：
Alblas J
影响因子：
--
作者：
Fulda S;Gorman AM;Hori O;Samali A
通讯作者：
Samali A
影响因子：
5.6
作者：
Bastidas-Coral, Angela P.;Hogervorst, Jolanda M. A.;Bakker, Astrid D.
通讯作者：
Bakker, Astrid D.
影响因子：
5.8
作者：
Kohno, Yusuke;Lin, Tzuhua;Goodman, Stuart B.
通讯作者：
Goodman, Stuart B.