Intracerebroventricular Administration of Leptin-Induced Apoptosis in the Rat Small Intestinal Mucosa
Intracerebroventricular Administration of Leptin-Induced Apoptosis in the Rat Small Intestinal Mucosa
复制标题
脑室内注射瘦素诱导大鼠小肠粘膜细胞凋亡
DOI：
10.1177/153537020322801022
复制
发表时间：
2003
影响因子：
3.2
通讯作者：
K. Fujimoto
中科院分区：
文献类型：
--
作者：
H. Utsumi;R. Iwakiri;Bin Wu;T. Fujise;Hiroyuki Sakata;R. Shimoda;S. Amemori;S. Tsunada;A. Ootani;K. Fujimoto
瘦素及其受体在胃和小肠的定位已有报道。尽管瘦素是一种调节食欲和脂肪相关代谢的激素，但它们的功能仍不清楚。小肠是调节新陈代谢的重要器官之一。本研究旨在探讨瘦素对小肠粘膜细胞凋亡的调节作用。DNA片段百分率、电泳法、原位末端标记法、半胱氨酸天冬氨酸氨基转移酶-3蛋白免疫印迹法检测肠上皮细胞凋亡。分别于注射后0、3、6、12、24小时检测大鼠空肠和回肠黏膜细胞凋亡情况。大鼠在自由喂养和禁食24小时后进行测试，以排除瘦素的厌食作用。Leptin按2 0 0μg/只大鼠腹腔注射，8μg/只大鼠第三脑室(Icv)注射。在自由喂养和禁食24小时的大鼠中，8μg/只的瘦素在侧脑室注射后3小时和6小时均能显著诱导小肠细胞的凋亡。这种细胞凋亡的增加并不能通过切断迷走神经来减弱。Ip瘦素2 0 0μg/只后12h和2 4h，肠上皮细胞凋亡率增加。ICV大鼠较IP大鼠出现细胞凋亡增加高峰的时间提前。瘦素诱导大鼠空肠和回肠黏膜细胞凋亡，提示瘦素可能通过调节肠道细胞凋亡来调控肠道功能。
The localization of leptin and leptin receptors in the stomach and small intestine has been reported. Their function is still unknown, although leptin is a hormone that regulates appetite and fat-related metabolism. The small intestine is one of the important organs for regulating metabolism. The purpose of the present study was to investigate whether leptin regulates apoptosis in the small intestinal mucosa. Intestinal apoptosis was evaluated by percent fragmented DNA, electrophoresis, TUNEL staining, and western blotting analysis of caspase-3. Mucosal apoptosis in the rat jejunum and Ileum was evaluated at 0, 3, 6, 12, and 24 hrs after injection. Rats were tested after ad libitum feeding and 24-hr fasting to exclude the anorectic effect of leptin. Leptin was injected intraperitoneally (ip) at a dose of 200 μg/rat and infused into the rat third cerebroventricle (icv) at a dose of 8 μg/rat. Leptin at a dose of 8 μg/rat significantly induced intestinal apoptosis in the small intestine at 3 and 6 hrs after icv administration in both ad libitum feeding and 24-hr fasted rats. This increase in apoptosis was not attenuated by vagotomy. Intestinal apoptosis increased 12 and 24 hrs after ip injection of leptin at a dose of 200 μg/rat. The peak of the increase in apoptosis in icv rats appeared earlier than that in ip rats. Leptin induced jejunal and ileal mucosal apoptosis in the rat, indicating that leptin might control intestinal function through the regulation of intestinal apoptosis.
影响因子：
1.9
作者：
Iwakiri, R;Gotoh, Y;Aw, TY
通讯作者：
Aw, TY
DOI：
10.1073/pnas.94.16.8878
发表时间：
1997-08-05
影响因子：
11.1
作者：
Halaas, JL;Boozer, C;Friedman, JM
通讯作者：
Friedman, JM
DOI：
10.1006/bbrc.1997.8193
发表时间：
1998-03-06
影响因子：
3.1
作者：
Potter, JJ;Womack, L;Anania, FA
通讯作者：
Anania, FA