The expression of epidermal antigens in Xenopus laevis.
The expression of epidermal antigens in Xenopus laevis.
复制标题
非洲爪蟾表皮抗原的表达。
作者：
K. Itoh;A. Yamashita;H. Kubota
制备了5种爪蟾胚胎表皮特异性单克隆抗体。表皮特异性抗体被用来调查在胚胎和幼虫发育过程中的表皮抗原的时空表达。在幼虫阶段被抗体识别的细胞如下：所有的外表皮细胞和粘固腺细胞都被称为XEPI-1的抗体识别，所有的外和内表皮细胞，除了粘固腺细胞，都被XEPI-2抗体识别，大的粘液颗粒和外表皮细胞的顶侧，除了纤毛表皮细胞，大粘液颗粒和基底膜被XEPI-4抗体识别，外表皮细胞中含有的小粘液颗粒以及细胞外基质被称为XEPI-5的抗体识别。所有的表皮抗原，除了XEPI-4，首先检测到的晚期原肠胚或早期神经胚的表皮区域。XEPI-4抗原首先在26期尾芽胚胎中检测到。这些抗原在胚胎发育过程中的任何时候都没有被神经组织表达。只有XEPI-2抗原在变态后继续表达，而其他抗原的表达在变态期间或之前消失。这些抗体的特异性使我们能够将表皮细胞在早期表皮发育中分为四种类型。四种类型的表皮细胞是（1）含有小粘液颗粒的外表皮细胞，（2）纤毛表皮细胞，（3）含有大粘液颗粒的外表皮细胞和（4）内感觉细胞。
Five kinds of monoclonal antibodies that are specific for the epidermis of Xenopus embryos were produced. Epidermis-specific antibodies were used to investigate the spatial and temporal expressions of epidermal antigens during embryonic and larval development. The cells that were recognized by the antibodies at the larval stage are as follows: all of the outer epidermal cells and cement gland cells were recognized by the antibody termed XEPI-1, all of the outer and inner epidermal cells, except the cement gland cells, were recognized by XEPI-2 antibody, the large mucus granules and the apical side of the outer epidermal cells, except for the ciliated epidermal cells, were recognized by XEPI-3 antibody, the large mucus granules and basement membrane were recognized by XEPI-4 antibody, and the small mucus granules contained in the outer epidermal cells as well as extracellular matrices were recognized by the antibody termed XEPI-5. All of the epidermal antigens, except XEPI-4, were first detected in the epidermal region of the late gastrula or early neurula. The XEPI-4 antigen was first detected in stage-26 tail-bud embryos. None of these antigens were expressed by the neural tissues at any time during embryonic development. Only the XEPI-2 antigen continued to be expressed after metamorphosis, while the expression of the other antigens disappeared during or before metamorphosis. The specificity of the antibodies allowed us to classify the epidermal cells into four types in early epidermal development. The four types of epidermal cells are (1) the outer epidermal cells that contain small mucus granules, (2) the ciliated epidermal cells, (3) the outer epidermal cells that contain large mucus granules and (4) the inner sensorial cells.
非洲爪蟾胚胎中神经细胞粘附分子（NCAM）的诱导。
影响因子：
2.7
作者：
Jacobson,M;Rutishauser,U
通讯作者：
Rutishauser,U
非洲爪蟾胚胎中神经细胞粘附分子的表达。
影响因子：
2.7
作者：
Balak,K;Jacobson,M;Sunshine,J;Rutishauser,U
通讯作者：
Rutishauser,U