Circadian regulation of glutamate release pathways shapes synaptic throughput in the brainstem nucleus of the solitary tract (NTS)
Circadian regulation of glutamate release pathways shapes synaptic throughput in the brainstem nucleus of the solitary tract (NTS)
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谷氨酸释放途径的昼夜节律调节影响孤束脑干核（NTS）的突触吞吐量
DOI：
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
J. H. Peters
中科院分区：
文献类型：
--
作者：
Forrest J. Ragozzino;BreeAnne Peterson;I. Karatsoreos;J. H. Peters
自主反射通路的昼夜节律调节将生理功能与每日光周期配对。脑干孤束核（NTS）是自主神经系统节律控制的关键候选者。在这里，我们研究了昼夜调节NTS神经传递和突触的吞吐量，采用膜片钳电生理学在小鼠脑干切片。我们发现，自发的定量谷氨酸释放到NTS神经元表现出很强的昼夜节律性，释放率最高，在光明的阶段和最低的黑暗中，这足以驱动白天/黑夜的差异组成性突触后动作电位放电。与此相反，传入诱发的动作电位吞吐量在黑暗中增强，在光下减少。传入驱动的同步释放途径表现出类似的释放概率下降，这并不能解释夜间突触吞吐量的增强。然而，突触后膜特性的分析揭示了电导的昼夜变化;当再加上谷氨酸释放途径的昼夜变化时，调节了亮相和暗相之间的突触通量。这些协调的突触前/突触后变化编码对突触性能的细微控制，并将NTS动作电位放电和迷走神经吞吐量与一天中的时间配对。
Circadian regulation of autonomic reflex pathways pairs physiological function with the daily light cycle. The brainstem nucleus of the solitary tract (NTS) is a key candidate for rhythmic control of the autonomic nervous system. Here we investigated circadian regulation of NTS neurotransmission and synaptic throughput using patch-clamp electrophysiology in brainstem slices from mice. We found that spontaneous quantal glutamate release on to NTS neurons showed strong circadian rhythmicity, with the highest rate of release during the light phase and the lowest in the dark, that were sufficient to drive day / night differences in constitutive postsynaptic action potential firing. In contrast, afferent-evoked action potential throughput was enhanced during the dark and diminished in the light. Afferent-driven synchronous release pathways showed a similar decrease in release probability that did not explain the enhanced synaptic throughput during the night. However, analysis of postsynaptic membrane properties revealed diurnal changes in conductance; which, when coupled with the circadian changes in glutamate release pathways, tuned synaptic throughput between the light and dark phases. These coordinated pre- / postsynaptic changes encode nuanced control over synaptic performance and pair NTS action potential firing and vagal throughput with time of day.
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影响因子：
2.5
作者：
Itri, J;Michel, S;Colwell, CS
通讯作者：
Colwell, CS
影响因子：
2.5
作者：
Michel, S;Itri, J;Colwell, CS
通讯作者：
Colwell, CS
影响因子：
10.8
作者：
Hu, Kun;Scheer, Frank A. J. L.;Shea, Steven A.
通讯作者：
Shea, Steven A.
DOI：
10.1152/ajpendo.00196.2011
发表时间：
2011-11
期刊：
American journal of physiology. Endocrinology and metabolism
影响因子：
--
作者：
Guo Zhang;D. Cai
通讯作者：
Guo Zhang;D. Cai
自由运行的昼夜呼吸节律被视交叉上核病变消除。
DOI：
10.1152/japplphysiol.00211.2020
发表时间：
2020
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Purnell,BentonS;Buchanan,GordonF
通讯作者：
Buchanan,GordonF