Community dynamics and functional characteristics of naphthalene-degrading populations in contaminated surface sediments and hypoxic/anoxic groundwater.
Community dynamics and functional characteristics of naphthalene-degrading populations in contaminated surface sediments and hypoxic/anoxic groundwater.
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在受污染的表面沉积物和低氧/缺氧地下水中萘降解种群的社区动态和功能特征。
DOI：
10.1111/1462-2920.14309
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发表时间：
2018-10
影响因子：
5.1
通讯作者：
Madsen E
中科院分区：
文献类型：
--
作者：
Wilhelm RC;Hanson BT;Chandra S;Madsen E
早期在纽约州南格伦斯瀑布（South Glens Falls）对影响煤焦油污染地下水自然衰减的生物地球化学因素进行了研究，揭示了厌氧代谢和降解物与细菌种群之间营养相互作用的重要性。这两种现象的现场表征已被证明具有挑战性，但稳定同位素探测（SIP）、单细胞成像和霰弹枪宏基因组学的进步现在为它们的研究提供了与培养无关的工具。我们利用呼吸测定、二次离子质谱成像和散弹枪宏基因组学等方法，在六天的时间里通过污染表层沉积物中的微生物种群追踪了13c标记的萘中的碳，以解开基于污染物的营养网。将低氧/缺氧地下水中的污染物暴露群落与含氧地表沉积物中的污染物暴露群落进行对比，以确定萘厌氧分解代谢的假定特征。总共有六种细菌负责萘的降解。在SIP孵育的早期阶段，铜单胞菌、拉尔斯顿菌和鞘单胞菌占主导地位，后期由窄养单胞菌和红球菌取代。宏基因组组装的基因组为这些时间变化背后的生态和功能特征提供了证据。在污染最严重的缺氧地下水中，有大量相同种类的窄养单胞菌和红球菌。在接触萘后，观察到嗜菌原生动物明显增加，尽管在细菌降解者和次级取食者群体之间转移的碳量微不足道。
Earlier research on the biogeochemical factors affecting natural attenuation in coal-tar contaminated groundwater, at South Glens Falls, NY, revealed the importance of anaerobic metabolism and trophic interactions between degrader and bacterivore populations. Field-based characterizations of both phenomena have proven challenging, but advances in stable isotope probing (SIP), single-cell imaging and shotgun metagenomics now provide cultivation-independent tools for their study. We tracked carbon from 13C-labeled naphthalene through microbial populations in contaminated surface sediments over six days using respiration assays, secondary ion mass spectrometry imaging and shotgun metagenomics to disentangle the contaminant-based trophic web. Contaminant-exposed communities in hypoxic/anoxic groundwater were contrasted with those from oxic surface sediments to identify putative features of anaerobic catabolism of naphthalene. In total, six bacteria were responsible for naphthalene degradation. Cupriavidus, Ralstonia and Sphingomonas predominated at the earliest stages of SIP incubations and were succeeded in later stages by Stenotrophomonas and Rhodococcus. Metagenome-assembled genomes provided evidence for the ecological and functional characteristics underlying these temporal shifts. Identical species of Stenotrophomonas and Rhodococcus were abundant in the most contaminated, anoxic groundwater. Apparent increases in bacterivorous protozoa were observed following exposure to naphthalene, though insignificant amounts of carbon were transferred between bacterial degraders and populations of secondary feeders.
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影响因子：
5.1
作者：
Frias-Lopez J;Thompson A;Waldbauer J;Chisholm SW
通讯作者：
Chisholm SW
影响因子：
4.4
作者：
Fuentes, Sebastian;Barra, Barbara;Seeger, Michael
通讯作者：
Seeger, Michael
影响因子：
7.4
作者：
Chandra, S;Smith, DR;Morrison, GH
通讯作者：
Morrison, GH
影响因子：
6.7
作者：
Chandra, Subhash;Pumphrey, Graham;Madsen, Eugene L.
通讯作者：
Madsen, Eugene L.
影响因子：
4.4
作者：
Griffiths, RI;Whiteley, AS;Bailey, MJ
通讯作者：
Bailey, MJ