A maze platform for the assessment of Caenorhabditis elegans behavior and learning.
A maze platform for the assessment of Caenorhabditis elegans behavior and learning.
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DOI：
10.1016/j.xpro.2021.100829
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发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Hsu AL
中科院分区：
文献类型：
--
作者：
Gourgou E;Hsu AL
迷宫被广泛用于研究动物的决策和空间学习。然而，它们很少被用来探索线虫的行为和训练-提高表现。该协议描述了一种高度可重复性的、低成本的迷宫平台，由标准的基于琼脂的线虫培养材料制成。它可以用来可靠地评估线虫的迷宫行为，我们最近应用了这个协议来建立多感觉学习。局限性包括在运动跟踪和区分学习形成和提取方面的挑战。有关本议定书的使用和执行的完整细节，请参阅。设计和3D打印迷宫模具和制造迷宫模板迷宫诱饵和插入线虫线虫记录线虫行为和分析数据需要格外小心的步骤提示迷宫被广泛用于研究动物决策和空间学习。然而，它们很少被用来探索线虫的行为和训练-提高表现。该协议描述了一种高度可重复性的、低成本的迷宫平台，由标准的基于琼脂的线虫培养材料制成。它可以用来可靠地评估线虫的迷宫行为，我们最近应用了这个协议来建立多感觉学习。局限性包括在运动跟踪和区分学习形成和提取方面的挑战。
Mazes are broadly used to investigate animal decision-making and spatial learning. However, they have been sparsely employed to explore C. elegans behavior and training-improved performance. This protocol describes a highly reproducible, low-cost maze platform, made of the standard, agar-based, nematode culturing material. It can be used to reliably assess C. elegans' maze behavior, and we have recently applied this protocol to establish multisensory learning. Limitations include challenges in locomotion tracking and in distinguishing learning formation versus retrieval. For complete details on the use and execution of this protocol, please refer to. Design and 3D-print the maze mold and fabricate the maze plates Bait the mazes and insert C. elegans nematodes Record nematode behavior and analyze data Tips on steps that require extra caution Mazes are broadly used to investigate animal decision-making and spatial learning. However, they have been sparsely employed to explore C. elegans behavior and training-improved performance. This protocol describes a highly reproducible, low-cost maze platform, made of the standard, agar-based, nematode culturing material. It can be used to reliably assess C. elegans' maze behavior, and we have recently applied this protocol to establish multisensory learning. Limitations include challenges in locomotion tracking and in distinguishing learning formation versus retrieval.
影响因子：
5.8
作者：
Gourgou E;Adiga K;Goettemoeller A;Chen C;Hsu AL
通讯作者：
Hsu AL