A calbindin‐immunoreactive cone bipolar cell type in the rabbit retina
A calbindin‐immunoreactive cone bipolar cell type in the rabbit retina
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兔视网膜中钙结合蛋白免疫反应性视锥双极细胞类型
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
S. Mills
中科院分区：
文献类型：
--
作者：
S. Massey;S. Mills
我们利用市售抗体和免疫细胞化学方法研究了钙结合蛋白calbindin在成年兔视网膜中的分布。最重标记的细胞是A型水平细胞，但B型水平细胞也被该抗体轻度标记。在水平细胞中，有一组小的、标记良好的体细胞，我们已经将其确定为ON锥双极细胞的一个子集。此外，一些宽视场无毛细胞和一些大神经节细胞也被标记为钙结合蛋白。calbinindin双极细胞形成一个规则的马赛克，峰值密度约为1,700个细胞/mm2，在外围下降到550个细胞/mm2。它们约占锥体双极细胞的十二分之一，并且在紧靠杆状双极末端的内丛状层的第4层下深处狭窄地分层。使用蛋白激酶C （PKC）抗体的双标签实验表明，钙结合蛋白双极细胞与棒状双极细胞完全不同。杆状双极细胞的数量超过calbinin锥形双极细胞的四到五倍。进一步的双标记实验表明，钙结合蛋白双极细胞也被标记为恢复。calbinindin双极细胞与AII腺分泌细胞偶联良好，约占AII偶联双极细胞的23%。这表明有三到四种额外的ON锥双极细胞类型与所有的无突细胞偶联。本文描述的calbindin锥形双极细胞与重建的锥形双极细胞具有许多相似的特征，后者与AII amacrine细胞形成最多的间隙连接（Strettoi et al. [1994] J. Comp. Neurol. 347:139-149）。我们得出的结论是，这些不同的方法提供了相同的锥双极细胞类型的互补描述。calbindin抗体定义了兔视网膜锥体双极细胞的一个亚群。这个亚群中的细胞几乎肯定是锥状双极细胞中最深的。钙结合蛋白锥双极细胞的紧密分层表明，内部网状层按深度分层，在膜下b的宽分区内具有狭窄的功能分区，在此处理ON信号。calbindin锥双极细胞和AII腺分泌细胞之间的偶联强度表明该途径在暗斑条件下起主要作用。©1996 Wiley‐Liss, Inc。
We have studied the distribution of the calcium‐binding protein calbindin in the adult rabbit retina by using a commercially available antibody and immunocytochemical methods. The most heavily labeled cells are A‐type horizontal cells, but B‐type horizontal cells are also lightly labeled by this antibody. Among the horizontal cells, there is a mosaic of small, well‐labeled somata, which we have identified as a subset of ON cone bipolar cells. In addition, some wide‐field amacrine cells and a few large ganglion cells are also labeled for calbindin. The calbindin bipolar cells form a regular mosaic with a peak density of approximately 1,700 cells/mm2, falling to 550 cells/mm2 in the periphery. They account for about one‐twelfth of cone bipolar cells, and they are narrowly stratified deep in sublamina 4 of the inner plexiform layer immediately above the rod bipolar terminals. Double‐label experiments using an antibody to protein kinase C (PKC) indicate that the calbindin bipolar cells are completely distinct from the population of rod bipolar cells. Rod bipolar cells outnumber the calbindin cone bipolar cells by a factor of four to five. Further double‐label experiments show that the calbindin bipolar cells are also labeled for recovering. The calbindin bipolar cells are well coupled to AII amacrine cells, and they account for roughly 23% of the AII coupled bipolar cells. This suggests that there are three to four additional ON cone bipolar cell types that are coupled to AII amacrine cells. The calbindin cone bipolar cell described in this paper shares many characteristics with a reconstructed cone bipolar cell that forms the most gap junctions with AII amacrine cells (Strettoi et al. [1994] J. Comp. Neurol. 347:139–149). We conclude that these different methodologies provide complementary descriptions of the same cone bipolar cell type. The calbindin antibody defines a subset of cone bipolar cells in the rabbit retina. The cells in this subset are almost certainly the deepest of the cone bipolar cells. The tight stratification of the calbindin cone bipolar cell suggests that the inner plexiform layer is stratified according to depth, with narrow functional divisions within the broad partition of sublamina b, where ON signals are processed. The strength of coupling between the calbindin cone bipolar cells and AII amacrine cells suggests this pathway plays a major role under scotopic conditions. © 1996 Wiley‐Liss, Inc.
DOI：
10.1098/rstb.1990.0202
发表时间：
1990-12-29
影响因子：
6.3
作者：
COHEN, E;STERLING, P
通讯作者：
STERLING, P
DOI：
10.1098/rstb.1990.0201
发表时间：
1990-12-29
影响因子：
6.3
作者：
COHEN, E;STERLING, P
通讯作者：
STERLING, P
脊椎动物视网膜中功能神经回路的结构。
影响因子：
4.4
作者：
Kolb,H
通讯作者：
Kolb,H