Cell surface expression of 27C7 by neonatal rat olfactory ensheathing cells in situ and in vitro is independent of axonal contact
Cell surface expression of 27C7 by neonatal rat olfactory ensheathing cells in situ and in vitro is independent of axonal contact
复制标题
新生大鼠嗅鞘细胞原位和体外的 27C7 细胞表面表达不依赖于轴突接触
DOI：
10.1007/s00418-011-0796-0
复制
发表时间：
2011
影响因子：
2.3
通讯作者：
K Wewetzer
中科院分区：
文献类型：
--
作者：
Brandes G;Khayami M;Peck C-T;Baumgärtner W;Bugday H;K Wewetzer
嗅鞘细胞是嗅系统的类雪旺细胞的神经胶质细胞，移植到受损的中枢神经系统后可促进神经再生。然而，与密切相关的雪旺细胞相比，嗅鞘细胞的生物学特性仍然是零碎的。这是因为嗅鞘细胞特异性标志物的表达受到复杂的调控，复杂的超微结构分析对确定其定位至关重要。例如，作为原型OEC标志物的p75神经营养因子受体（p75 NTR）仅由少量新生大鼠OEC原位表达。主要群体在解离后在其表面上携带O 4阳性轴突片段，并在培养期间上调p75 NTR（Wewetzer等，Glia 49：577-587，2005）。在本研究中，我们调查了是否细胞表面决定簇27 C7，定义的单克隆抗体雪旺细胞，也表达新生大鼠嗅鞘细胞在原位和体外。嗅球的原代细胞悬浮液显示p75 NTR阴性和p75 NTR阳性嗅鞘细胞均表达27 C7，而未成熟的少突胶质细胞和星形胶质细胞则没有任何27 C7标记。这一发现以及粘膜中嗅神经束内OEC表达27 C7而不表达p75 NTR的发现表明，27 C7在整个OEC群体中原位表达。维持嗅鞘细胞在嗅觉神经元的情况下，在器官型切片培养上调p75 NTR，但没有改变27 C7的表达。它的结论是，27 C7不像p75 NTR组成型OECs表达，因此，可能是一个有用的标记物，用于表征新生儿OECs在原位和体外。
Olfactory ensheathing cells (OECs) are Schwann cell-like glial cells of the olfactory system that promote neural regeneration after transplantation into the injured central nervous system. Compared to the closely related Schwann cells, however, the biological characterization of OECs has remained fragmentary. This is due to the fact that the expression of OEC-specific markers is subject to complex regulation and that intricate ultrastructural analysis is essential to determine their localization. The p75 neurotrophin receptor (p75NTR) as the prototype OEC marker, for example, is only expressed by a minor population of neonatal rat OECs in situ. The major population carries O4-positive axonal fragments on their surface after dissociation and up-regulates p75NTRduring culturing (Wewetzer et al. in Glia 49:577–587, 2005). In the present study, we investigated whether the cell surface determinant 27C7, defined by a monoclonal antibody to Schwann cells, is also expressed by neonatal rat OECs in situ and in vitro. Primary cell suspensions of the olfactory bulb displayed 27C7 expression of both p75NTR-negative and p75NTR-positive OECs, while immature oligodendrocytes and astrocytes were devoid of any 27C7 labeling. This together with the finding that the intrafascicular OECs of the olfactory nerves in the mucosa expressed 27C7 but not p75NTR, suggests that 27C7 was expressed by the entire OEC population in situ. Maintenance of OECs in the absence of olfactory neurons in organotypic slice culture up-regulated p75NTRbut did not alter 27C7 expression. It is concluded that 27C7 unlike p75NTRis constitutively expressed by OECs and may, therefore, be a useful marker for characterization of neonatal OECs in situ and in vitro.
登录
查看更多内容
轴突信号传导和嗅觉鞘细胞的形成：一个假设。
影响因子：
--
作者：
K. Wewetzer;G. Brandes
通讯作者：
G. Brandes
吖啶橙与大鼠关节软骨中蛋白多糖的反应
DOI：
--
发表时间：
1990
期刊：
The Histochemical Journal
影响因子：
--
作者：
G. Brandes;E. Reale
通讯作者：
E. Reale
影响因子：
3.3
作者：
Asan, E;Langenhan, T;Carroll, P
通讯作者：
Carroll, P
单克隆抗体调节神经生长因子与 PC12 细胞的相互作用。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chandler,CE;Parsons,LM;Hosang,M;Shooter,EM
通讯作者：
Shooter,EM
切片培养物作为研究内嗅-海马相互作用的模型
影响因子：
3.5
作者：
B. Heimrich;M. Frotscher
通讯作者：
M. Frotscher