Stimulation of lateral hypothalamic glutamate and acetylcholine efflux by nicotine: implications for mechanisms of nicotine-induced activation of orexin neurons.
Stimulation of lateral hypothalamic glutamate and acetylcholine efflux by nicotine: implications for mechanisms of nicotine-induced activation of orexin neurons.
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DOI：
10.1111/j.1471-4159.2010.06666.x
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发表时间：
2010-05
影响因子：
4.7
通讯作者：
Fadel J
中科院分区：
文献类型：
--
作者：
Pasumarthi RK;Fadel J
下丘脑是尼古丁作用的主要靶点。我们以前已经表明，急性全身尼古丁治疗诱导Fos表达在外侧下丘脑和穹窿周围区（LH/PFA），食欲素/下丘脑泌素神经元特别敏感。然而，尚未描述LH/PFA中急性尼古丁治疗的神经化学相关性。解剖学研究表明，该区域接受来自胆碱能、多巴胺能和GABA能端脑区域的传入，表明这些神经递质在介导尼古丁对稳态现象（如唤醒和食欲）影响的下丘脑组分中具有潜在作用。在这里，我们在体内微透析，以确定急性全身或局部尼古丁对谷氨酸，乙酰胆碱和GABA流出的LH/PFA的大鼠。局部给药尼古丁显着增加乙酰胆碱和谷氨酸，但不是GABA，在LH/PFA。因此，我们进一步测试的作用，传入来源的谷氨酸和乙酰胆碱介导急性尼古丁诱导的食欲素神经元的激活单方面病变的前额叶皮层或基底前脑胆碱能区。损伤的动物表现出减少Fos阳性食欲素神经元尼古丁治疗。这些数据表明，乙酰胆碱和谷氨酸可能介导急性尼古丁对下丘脑神经元，包括食欲素/下丘脑泌素细胞的活动的影响。慢性尼古丁引起的该区域胆碱能或多巴胺能传递的变化可能导致LH/PFA神经元介导的功能的长期改变，包括进食和唤醒。
The hypothalamus is a prominent target of nicotine action. We have previously shown that acute systemic nicotine treatment induces Fos expression in the lateral hypothalamus and perifornical area (LH/PFA), with orexin/hypocretin neurons being particularly responsive. However, the neurochemical correlates of acute nicotine treatment in the LH/PFA have not been described. Anatomical studies have revealed that this area receives afferents from cholinergic, glutamatergic and GABAergic telencephalic brain regions, suggesting a potential role for these neurotransmitters in mediating the hypothalamic component of nicotine effects on homeostatic phenomena such as arousal and appetite. Here, we used in vivo microdialysis to determine the effect of acute systemic or local nicotine on glutamate, acetylcholine and GABA efflux in the LH/PFA of rats. Local administration of nicotine significantly increased ACh and glutamate, but not GABA, in the LH/PFA. Thus, we further tested the role of afferent sources of glutamate and acetylcholine in mediating acute nicotine-induced activation of orexin neurons by unilaterally lesioning the prefrontal cortex or basal forebrain cholinergic regions. Lesioned animals showed reduced Fos-positive orexin neurons following nicotine treatment. These data suggest that both ACh and glutamate may mediate the effects of acute nicotine on the activity of hypothalamic neurons, including orexin/hypocretin cells. Changes in cholinergic or glutamatergic transmission in this region with chronic nicotine may contribute to long-term alterations in functions mediated by LH/PFA neurons, including feeding and arousal.
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影响因子：
3.3
作者：
Fadel, J;Pasumarthi, R;Reznikov, LR
通讯作者：
Reznikov, LR
影响因子：
16.2
作者：
Li, Y;Gao, XB;van den Pol, AN
通讯作者：
van den Pol, AN
DOI：
10.1073/pnas.0808023105
发表时间：
2008-12-09
影响因子：
11.1
作者：
Hollander, Jonathan A.;Lu, Qun;Kenny, Paul J.
通讯作者：
Kenny, Paul J.
影响因子：
5.3
作者：
Jo, YH;Wiedl, D;Role, LW
通讯作者：
Role, LW
影响因子：
5.3
作者：
Acuna-Goycolea, C;Li, Y;van den Pol, AN
通讯作者：
van den Pol, AN