Dexmedetomidine attenuates cerebral ischemia/reperfusion injury in neonatal rats by inhibiting TLR4 signaling.
Dexmedetomidine attenuates cerebral ischemia/reperfusion injury in neonatal rats by inhibiting TLR4 signaling.
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DOI：
10.1177/0300060518781382
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Peng X
中科院分区：
文献类型：
--
作者：
Cheng J;Zhu P;Qin H;Li X;Yu H;Yu H;Peng X
右美托咪定在缺血/再灌注损伤中通过抑制toll样受体（TLR） 4的表达发挥多器官保护作用。本研究探讨了右美托咪定的神经保护作用是否可以被TLR4激动剂脂多糖阻断。我们通过双侧颈动脉闭塞20分钟后再灌注2小时建立了新生儿Sprague-Dawley大鼠脑缺血再灌注模型。将大鼠分为假手术、缺血/再灌注、缺血/再灌注前给予右美托咪定（10µg/kg）、缺血/再灌注前给予右美托咪定（10µg/kg）和脂多糖（500µg/kg）处理四组。采用苏木精和伊红染色检测脑组织损伤，采用实时荧光定量PCR和western blot检测脑组织TLR4表达。右美托咪定预处理降低了海马缺血诱导的CA3区形态学变化，下调了TLR4的表达，但这些神经保护作用被TLR4激动剂脂多糖联合治疗部分阻断。我们的研究结果表明，右美托咪定对新生大鼠脑缺血再灌注模型脑TLR4表达的抑制与神经保护作用有关。
The sedative dexmedetomidine plays a role in multi-organ protection by inhibiting toll-like receptor (TLR) 4 expression in ischemia/reperfusion injury. The present study investigated whether the neuroprotective effects of dexmedetomidine could be blocked by the TLR4 agonist lipopolysaccharide. We established a cerebral ischemia/reperfusion model in neonatal Sprague-Dawley rats through bilateral carotid artery occlusion for 20 minutes followed by a 2-hour reperfusion. Rats were assigned to four groups: Sham operation, ischemia/reperfusion, ischemia/reperfusion preceded by dexmedetomidine treatment (10 µg/kg), and ischemia/reperfusion preceded by dexmedetomidine (10 µg/kg) and lipopolysaccharide (500 µg/kg) treatments. Cerebral tissue injury was assessed by hematoxylin and eosin staining, and cerebral TLR4 expression was evaluated by real-time PCR and western blot. Pretreatment with dexmedetomidine reduced ischemia-induced morphological changes in the hippocampal CA3 region and downregulated TLR4 expression, but these neuroprotective effects were partially blocked by co-treatment with the TLR4 agonist lipopolysaccharide. Our results indicate that inhibition of cerebral TLR4 expression is related to the neuroprotective effects of dexmedetomidine in this neonatal rat cerebral ischemia/reperfusion model.
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影响因子：
4.4
作者：
Marquardt, L;Ruf, A;Grau, AJ
通讯作者：
Grau, AJ
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Tao T
影响因子：
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作者：
Arslan F;Keogh B;McGuirk P;Parker AE
通讯作者：
Parker AE
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5.3
作者：
Zhao P;Wang J;He L;Ma H;Zhang X;Zhu X;Dolence EK;Ren J;Li J
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Li J
TOLL样受体-4可以协调肾脏对肾脏缺血/再灌注损伤的先天免疫反应。
影响因子：
3.7
作者：
Pulskens, Wilco P.;Teske, Gwendoline J.;Butter, Loes M.;Roelofs, Joris J.;van der Poll, Tom;Florquin, Sandrine;Leemans, Jaklien C.
通讯作者：
Leemans, Jaklien C.