NMR studies on the interactions between yeast Vta1 and Did2 during the multivesicular bodies sorting pathway.
NMR studies on the interactions between yeast Vta1 and Did2 during the multivesicular bodies sorting pathway.
复制标题
酵母 Vta1 和 Did2 在多泡体分选途径中相互作用的 NMR 研究。
DOI：
10.1038/srep38710
复制
发表时间：
2016-12-07
影响因子：
4.6
通讯作者：
Cao C
中科院分区：
文献类型：
--
作者：
Shen J;Yang Z;Wang J;Zhao B;Lan W;Wang C;Zhang X;Wild CJ;Liu M;Xu Z;Cao C
As an AAA-ATPase, Vps4 is important for function of multivesicular bodies (MVB) sorting pathway, which involves in cellular phenomena ranging from receptor down-regulation to viral budding to cytokinesis. The activity of Vps4 is stimulated by the interactions between Vta1 N-terminus (named as Vta1NTD) and Did2 fragment (176–204 aa) (termed as Did2176–204) or Vps60 (128–186 aa) (termed as Vps60128–186). The structural basis of how Vta1NTD binds to Did2176–204 is still unclear. To address this, in this report, the structure of Did2176–204 in complex with Vta1NTD was determined by NMR techniques, demonstrating that Did2176–204 interacts with Vta1NTD through its helix α6′ extending over the 2nd and the 3rd α-helices of Vta1NTD microtubule interacting and transport 1 (MIT1) domain. The residues within Did2176–204 helix α6′ in the interface make up of an amino acid sequence as E192′xxL195′xxR198′L199′xxL202′R203′, identical to type 1 MIT-interacting motif (MIM1) (D/E)xxLxxRLxxL(K/R) of CHMP1A180–196 observed in Vps4-CHMP1A complex structure, indicating that Did2 binds to Vta1NTD through canonical MIM1 interactions. Moreover, the Did2 binding does not result in Vta1NTD significant conformational changes, revealing that Did2, similar to Vps60, enhances Vta1 stimulation of Vps4 ATPase activity in an indirect manner.
登录
查看更多内容
DOI：
10.1083/jcb.200508166
发表时间：
2006-02-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Azmi I;Davies B;Dimaano C;Payne J;Eckert D;Babst M;Katzmann DJ
通讯作者：
Katzmann DJ
ESCRT系统在古细胞分裂中的作用。
DOI：
10.1126/science.1165322
发表时间：
2008-12-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Samson RY;Obita T;Freund SM;Williams RL;Bell SD
通讯作者：
Bell SD
影响因子：
56.9
作者：
Carlton, Jez G.;Martin-Serrano, Juan
通讯作者：
Martin-Serrano, Juan
影响因子：
17.3
作者：
Clore, GM;Gronenborn, AM
通讯作者：
Gronenborn, AM
影响因子：
2.7
作者：
DELAGLIO, F;GRZESIEK, S;BAX, A
通讯作者：
BAX, A