Distribution and morphology of calcitonin gene-related peptide and substance P immunoreactive axons in the whole-mount atria of mice.
Distribution and morphology of calcitonin gene-related peptide and substance P immunoreactive axons in the whole-mount atria of mice.
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DOI：
10.1016/j.autneu.2013.12.010
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发表时间：
2014-04
影响因子：
2.7
通讯作者：
Cheng, Zixi (Jack)
中科院分区：
文献类型：
--
作者：
Li, Liang;Hatcher, Jeffrey T.;Hoover, Donald B.;Gu, He;Wurster, Robert D.;Cheng, Zixi (Jack)
小鼠模型已被用于研究心脏感觉轴突在各种疾病状态中的作用。然而，心脏伤害性轴突在正常小鼠组织中的分布和形态结构尚未得到很好的表征。在这项研究中，来自FVB小鼠的整装心房用降钙素基因相关肽（CGRP）和P物质（SP）一抗处理，然后用二抗处理，然后用共聚焦显微镜检查。我们发现：（1）CGRP-IR轴突大束随主静脉进入心房，大束分叉成小束和单个轴突，在心外膜形成终末网和游离终末。曲张的CGRP-IR轴突与肌纤维紧密接触，部分CGRP-IR轴突在心内神经节（ICG）内的主神经元（PN）周围形成曲张。2)SP-IR轴突也被发现在心房的相同区域，连接到静脉，和心脏神经节内。与CGRP-IR轴突相似，这些SP-IR轴突在心房心外膜和心肌中形成终末网和游离终末。在ICG内，SP-IR轴突在PN周围形成曲张的末梢。然而，SP-IR神经纤维在心房中的丰富度低于CGRP-IR纤维。3)4）CGRP-IR和SP-IR常共定位于PN周围终末静脉曲张处。总的来说，我们的数据文件的分布模式和形态的CGRP-IR和SP-IR轴突和终端在不同区域的心房。这一发现为CGRP-IR和SP-IR轴突的研究提供了有用的信息，可为今后病理性重塑的研究提供参考。
The murine model has been used to investigate the role of cardiac sensory axons in various disease states. However, the distribution and morphological structures of cardiac nociceptive axons in normal murine tissues have not yet been well characterized. In this study, whole-mount atria from FVB mice were processed with calcitonin gene-related peptide (CGRP) and substance P (SP) primary antibodies followed by secondary antibodies, and then examined using confocal microscopy. We found: 1) Large CGRP-IR axon bundles entered the atria with the major veins, and these large bundles bifurcated into small bundles and single axons that formed terminal end-nets and free endings in the epicardium. Varicose CGRP-IR axons had close contacts with muscle fibers, and some CGRP-IR axons formed varicosities around principle neurons (PNs) within intrinsic cardiac ganglia (ICGs). 2) SP-IR axons also were found in the same regions of the atria, attached to veins, and within cardiac ganglia. Similar to CGRP-IR axons, these SP-IR axons formed terminal end-nets and free endings in the atrial epicardium and myocardium. Within ICGs, SP-IR axons formed varicose endings around PNs. However, SP-IR nerve fibers were less abundant than CGRP-IR fibers in the atria. 3) None of the PNs were CGRP-IR or SP-IR. 4) CGRP-IR and SP-IR often colocalized in terminal varicosities around PNs. Collectively, our data document the distribution pattern and morphology of CGRP-IR and SP-IR axons and terminals in different regions of the atria. This knowledge provides useful information for CGRP-IR and SP-IR axons that can be referred to in future studies of pathological remodeling.
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影响因子：
4.5
作者：
Gillespie, JI
通讯作者：
Gillespie, JI
DOI：
10.1016/0165-1838(94)00182-j
发表时间：
1995-06-25
期刊：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM
影响因子：
--
作者：
HARDWICK, JC;MAWE, GM;PARSONS, RL
通讯作者：
PARSONS, RL
影响因子：
2.7
作者：
Collins, Tony J.
通讯作者：
Collins, Tony J.
影响因子：
3
作者：
FRANCOCERECEDA, A;HENKE, H;FISCHER, JA
通讯作者：
FISCHER, JA
影响因子：
3.3
作者：
Hoover, D. B.;Isaacs, E. R.;Armour, J. A.
通讯作者：
Armour, J. A.