Myocardial matrix-polyethylene glycol hybrid hydrogels for tissue engineering.
Myocardial matrix-polyethylene glycol hybrid hydrogels for tissue engineering.
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DOI：
10.1088/0957-4484/25/1/014011
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发表时间：
2014-01-10
期刊：
影响因子：
3.5
通讯作者：
Christman KL
中科院分区：
文献类型：
--
作者：
Grover GN;Rao N;Christman KL
类似于其他基于蛋白质的水凝胶，源自脱细胞组织的基于细胞外基质（ECM）的水凝胶具有窄范围的机械性质并且快速降解。这些水凝胶含有天然细胞粘附位点，形成类似于天然ECM的纳米纤维网络，并且是可生物降解的。在这项研究中，我们通过将聚乙二醇（PEG）纳入ECM网络来扩展这些类型材料的特性。我们使用脱细胞心肌基质作为组织特异性ECM衍生水凝胶的实例。心肌基质-PEG杂化物通过两种不同的方法合成，用胺反应性PEG-星形和两种不同的多臂PEG-丙烯酸酯的光诱导自由基聚合交联蛋白质。我们表明，这两种方法允许共轭PEG心肌基质的凝胶电泳和红外光谱。扫描电子显微镜显示，杂化材料仍然包含类似于未修饰的心肌基质的纳米纤维网络，并且通过PEG掺入和PEG分子量的方法改变纤维直径。PEG结合还降低了体外酶降解速率，并增加了材料刚度。用胺反应性PEG合成的混合物具有30分钟的凝胶化速率，类似于未修饰的心肌基质，并且PEG的掺入没有阻止细胞粘附和通过水凝胶的迁移，因此提供了具有在体内降解更慢的可注射ECM水凝胶的可能性。光聚合的自由基系统在照射后4分钟内胶凝，允许3D封装和细胞培养，不像柔软的未修饰的心肌基质。这项工作表明，将PEG掺入基于ECM的水凝胶可以扩展材料特性，从而为体外和体内应用开辟了新的可能性。
Similar to other protein-based hydrogels, extracellular matrix (ECM) based hydrogels, derived from decellularized tissues, have a narrow range of mechanical properties and are rapidly degraded. These hydrogels contain natural cellular adhesion sites, form nanofibrous networks similar to native ECM, and are biodegradable. In this study, we expand the properties of these types of materials by incorporating poly(ethylene glycol) (PEG) into the ECM network. We use decellularized myocardial matrix as an example of a tissue specific ECM derived hydrogel. Myocardial matrix-PEG hybrids were synthesized by two different methods, cross-linking the proteins with an amine-reactive PEG-star and photo-induced radical polymerization of two different multi-armed PEG-acrylates. We show that both methods allow for conjugation of PEG to the myocardial matrix by gel electrophoresis and infrared spectroscopy. Scanning electron microscopy demonstrated that the hybrid materials still contain a nanofibrous network similar to unmodified myocardial matrix and that the fiber diameter is changed by the method of PEG incorporation and PEG molecular weight. PEG conjugation also decreased the rate of enzymatic degradation in vitro, and increased material stiffness. Hybrids synthesized with amine-reactive PEG had gelation rates of thirty minutes, similar to the unmodified myocardial matrix, and incorporation of PEG did not prevent cell adhesion and migration through the hydrogels, thus offering the possibility to have an injectable ECM hydrogel that degrades more slowly in vivo. The photo-polymerized radical systems gelled in four minutes upon irradiation allowing for 3D encapsulation and culture of cells, unlike the soft unmodified myocardial matrix. This work demonstrates PEG incorporation into ECM-based hydrogels can expand material properties, thereby opening up new possibilities for in vitro and in vivo applications.
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影响因子：
24
作者：
Singelyn, Jennifer M.;Sundaramurthy, Priya;Johnson, Todd D.;Schup-Magoffin, Pamela J.;Hu, Diane P.;Faulk, Denver M.;Wang, Jean;Mayle, Kristine M.;Bartels, Kendra;Salvatore, Michael;Kinsey, Adam M.;DeMaria, Anthony N.;Dib, Nabil;Christman, Karen L.
通讯作者：
Christman, Karen L.
影响因子：
3.8
作者：
Tibbitt, Mark W.;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.
影响因子：
3.1
作者：
DeQuach JA;Lin JE;Cam C;Hu D;Salvatore MA;Sheikh F;Christman KL
通讯作者：
Christman KL
影响因子：
6.2
作者：
Browning, Mary Beth;Russell, Brooke;Cosgriff-Hernandez, Elizabeth M.
通讯作者：
Cosgriff-Hernandez, Elizabeth M.
影响因子：
17.1
作者：
Seif-Naraghi SB;Singelyn JM;Salvatore MA;Osborn KG;Wang JJ;Sampat U;Kwan OL;Strachan GM;Wong J;Schup-Magoffin PJ;Braden RL;Bartels K;DeQuach JA;Preul M;Kinsey AM;DeMaria AN;Dib N;Christman KL
通讯作者：
Christman KL