Hydrogels as extracellular matrix mimics for 3D cell culture.
Hydrogels as extracellular matrix mimics for 3D cell culture.
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DOI：
10.1002/bit.22361
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发表时间：
2009-07-01
影响因子：
3.8
通讯作者：
Anseth, Kristi S.
中科院分区：
文献类型：
--
作者：
Tibbitt, Mark W.;Anseth, Kristi S.
越来越需要离体培养哺乳动物细胞的方法来研究细胞和组织生理学以及生长用于再生医学的替代组织。二维培养一直是典型的体外细胞培养的范例;然而，已经证明，当在三维环境中培养时，细胞表现得更天然。允许的合成水凝胶和促进的天然水凝胶作为三维细胞培养平台已经变得流行;然而，这两种系统都具有局限性。在这个角度来看，我们讨论了使用合成和天然水凝胶作为三维细胞培养的支架，以及合成水凝胶，将复杂的生化和机械线索作为模仿天然细胞外基质。最终，需要合成生物水凝胶混合物的进展，以提供研究细胞生理学和在生物体外制造组织的强大平台。
Methods for culturing mammalian cells ex vivo are increasingly needed to study cell and tissue physiology and to grow replacement tissue for regenerative medicine. Two-dimensional culture has been the paradigm for typical in vitro cell culture; however, it has been demonstrated that cells behave more natively when cultured in three-dimensional environments. Permissive, synthetic hydrogels and promoting, natural hydrogels have become popular as three-dimensional cell culture platforms; yet, both of these systems possess limitations. In this perspective, we discuss the use of both synthetic and natural hydrogels as scaffolds for three-dimensional cell culture as well as synthetic hydrogels that incorporate sophisticated biochemical and mechanical cues as mimics of the native extracellular matrix. Ultimately, advances in synthetic–biologic hydrogel hybrids are needed to provide robust platforms for investigating cell physiology and fabricating tissue outside of the organism.
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DOI：
10.1073/pnas.1735463100
发表时间：
2003-10-28
影响因子：
11.1
作者：
Levenberg, S;Huang, NF;Langer, R
通讯作者：
Langer, R
影响因子：
14
作者：
Lee, Soo-Hong;Moon, James J.;West, Jennifer L.
通讯作者：
West, Jennifer L.
影响因子：
--
作者：
Khire, Vaibhav S.;Benoit, Danielle S. W.;Bowman, Christopher N.
通讯作者：
Bowman, Christopher N.
DOI：
10.1073/pnas.0737381100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Lutolf, MP;Lauer-Fields, JL;Hubbell, JA
通讯作者：
Hubbell, JA
影响因子：
7.5
作者：
Howe, A;Aplin, AE;Juliano, RL
通讯作者：
Juliano, RL