DMNet: Dual-Stream Marker Guided Deep Network for Dense Cell Segmentation and Lineage Tracking.
DMNet: Dual-Stream Marker Guided Deep Network for Dense Cell Segmentation and Lineage Tracking.
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DOI:
10.1109/iccvw54120.2021.00375
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发表时间:
2021-10
期刊:
影响因子:
--
通讯作者:
Palaniappan K
中科院分区:
文献类型:
--
作者:
Bao R;Al-Shakarji NM;Bunyak F;Palaniappan K
Accurate segmentation and tracking of cells in microscopy image sequences is extremely beneficial in clinical diagnostic applications and biomedical research. A continuing challenge is the segmentation of dense touching cells and deforming cells with indistinct boundaries, in low signal-to-noise-ratio images. In this paper, we present a dual-stream marker-guided network (DMNet) for segmentation of touching cells in microscopy videos of many cell types. DMNet uses an explicit cell marker-detection stream, with a separate mask-prediction stream using a distance map penalty function, which enables supervised training to focus attention on touching and nearby cells. For multi-object cell tracking we use M2Track tracking-by-detection approach with multi-step data association. Our M2Track with mask overlap includes short term track-to-cell association followed by track-to-track association to re-link tracklets with missing segmentation masks over a short sequence of frames. Our combined detection, segmentation and tracking algorithm has proven its potential on the IEEE ISBI 2021 6th Cell Tracking Challenge (CTC-6) where we achieved multiple top three rankings for diverse cell types. Our team name is MU-Ba-US, and the implementation of DMNet is available at, http://celltrackingchallenge.net/participants/MU-Ba-US/.
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