In vivo high-content imaging and regression analysis reveal non-cell autonomous functions of Shroom3 during neural tube closure.
In vivo high-content imaging and regression analysis reveal non-cell autonomous functions of Shroom3 during neural tube closure.
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DOI：
10.1016/j.ydbio.2022.08.011
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发表时间：
2022-11
影响因子：
2.7
通讯作者：
Wallingford, John B.
中科院分区：
文献类型：
--
作者：
Baldwin, Austin T.;Kim, Juliana H.;Wallingford, John B.
在神经管闭合过程中，神经外胚层细胞收缩其顶端表面弯曲并将组织折叠成一个管，这将成为中枢神经系统。最近来自患有神经管缺陷的小鼠和人类的数据表明，神经管闭合所需的关键基因可以对细胞行为施加非细胞自主影响，但这些影响的性质仍然不清楚。在这里，我们将非洲爪蟾闭合神经管的组织尺度、高分辨率延时成像与多元回归模型相结合，我们发现内侧肌动蛋白积累在细胞附近非自主地驱动根尖收缩，而不仅仅是单个细胞。为了进一步探索这种效应，我们研究了马赛克脆化胚胎，并确定了顶端收缩蛋白Shroom3的自主和非自主作用。
During neural tube closure, neural ectoderm cells constrict their apical surfaces to bend and fold the tissue into a tube that will become the central nervous system. Recent data from mice and humans with neural tube defects suggest that key genes required for neural tube closure can exert non-cell autonomous effects on cell behavior, but the nature of these effects remains obscure. Here, we coupled tissue-scale, high-resolution time-lapse imaging of the closing neural tube of Xenopus to multivariate regression modeling, and we show that medial actin accumulation drives apical constriction non-autonomously in neighborhoods of cells, rather than solely in individual cells. To further explore this effect, we examined mosaic crispant embryos and identified both autonomous and non-autonomous effects of the apical constriction protein Shroom3.
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