Staphylococcal cassette chromosome mec containing a novel mec gene complex, B4.
Staphylococcal cassette chromosome mec containing a novel mec gene complex, B4.
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含有新型 mec 基因复合体 B4 的葡萄球菌盒式染色体 mec。
DOI：
10.1093/jac/dkab154
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发表时间：
2021-07-15
期刊：
影响因子：
--
通讯作者：
Friedrich AW
中科院分区：
文献类型：
--
作者：
Sabat AJ;Bathoorn E;Becker K;Akkerboom V;Miskoski M;Durfee T;Friedrich AW
目的描述表皮葡萄球菌mec B类复合体的一个新亚类。对格罗宁根大学医学中心（UMCG）患者血液感染中分离的4株表皮葡萄球菌进行了表型抗生素敏感性试验和WGS分析。序列分析显示UMCG335中存在一个新的葡萄球菌盒式染色体mec （SCCmec）结构。在这种结构中，质粒pUB110被整合到SCCmec IVc中，形成了一个新的SCCmec亚型IVUMCG335。在其他分离株UMCG364和UMCG341中分别发现了SCCmec IVc和质粒pUB110的拷贝，表明SCCmec IVUMCG335可能在UMCG进化而来。UMCG337的SCCmec包含一个新的mec复合体遗传组织（IS431-ΔmecR1-mecA-IS431-pUB110-IS431-ψIS1272），我们将其命名为B4。mec B类复合体的这一新亚类是由is431介导的包含质粒的DNA片段和mec复合体的大部分基因（IS1272除外）的反转产生的。由于UMCG337中的SCCmec组织与SCCmec IVUMCG335的序列倒置约10 kb，因此我们将其命名为SCCmec亚型IVUMCG337。分离株UMCG335和UMCG337分别携带SCCmec IVUMCG335和IVUMCG337，分别与一个限制性修饰系统和一个CRISPR-Cas系统关联，形成一个近70 kb的复合岛。我们的研究结果强调了IS431在SCCmec区域进化中的重要性。在SCCmec元素中发现的日益增加的遗传多样性对SCCmec分型方法提出了巨大的挑战，并突出了SCCmec命名法可能存在的困难。
To describe a new subclass of mec class B complex identified in Staphylococcus epidermidis. Four S. epidermidis isolates obtained from bloodstream infections in patients at University Medical Center Groningen (UMCG) were analysed by phenotypic antibiotic susceptibility testing and WGS. Sequence analysis revealed a new staphylococcal cassette chromosome mec (SCCmec) structure in isolate UMCG335. In this structure, plasmid pUB110 was found to be integrated into SCCmec IVc, creating a new SCCmec subtype, IVUMCG335. SCCmec IVc and a copy of plasmid pUB110 were found in other isolates, UMCG364 and UMCG341, respectively, indicating a probability that SCCmec IVUMCG335 could have evolved at the UMCG. SCCmec of UMCG337 contained a new genetic organization of the mec complex (IS431-ΔmecR1-mecA-IS431-pUB110-IS431-ψIS1272) that we have named B4. This new subclass of mec class B complex originated by IS431-mediated inversion of the DNA segment encompassing the plasmid and most of the genes of the mec complex with the exception of IS1272. As the SCCmec organization in UMCG337 differed by the inversion of an ∼10 kb sequence compared with SCCmec IVUMCG335, we have named it SCCmec subtype IVUMCG337. Isolates UMCG335 and UMCG337 carrying SCCmec IVUMCG335 and IVUMCG337, respectively, were associated with a restriction-modification system and a CRISPR-Cas system, creating a composite island of almost 70 kb. Our findings highlight the importance of IS431 in the evolution of the SCCmec region. The increasing genetic diversity identified in the SCCmec elements imposes a great challenge for SCCmec typing methods and highlights possible difficulties with the SCCmec nomenclature.
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影响因子：
4.8
作者：
Kaya H;Hasman H;Larsen J;Stegger M;Johannesen TB;Allesøe RL;Lemvigh CK;Aarestrup FM;Lund O;Larsen AR
通讯作者：
Larsen AR
影响因子：
4.2
作者：
Zong Z
通讯作者：
Zong Z
影响因子：
4.9
作者：
Sabat, Artur J.;Koeck, Robin;Friedrich, Alexander W.
通讯作者：
Friedrich, Alexander W.
影响因子：
3.9
作者：
Cortes, Marina F.;Botelho, Ana M. N.;Figueiredo, Agnes M. S.
通讯作者：
Figueiredo, Agnes M. S.
影响因子：
5.2
作者：
Urushibara, Noriko;Aung, Meiji Soe;Kobayashi, Nobumichi
通讯作者：
Kobayashi, Nobumichi