Endoglin is a novel endothelial cell specification gene.
Endoglin is a novel endothelial cell specification gene.
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DOI：
10.1016/j.scr.2011.08.006
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发表时间：
2012-01
影响因子：
1.2
通讯作者：
Bacanamwo, Methode
中科院分区：
文献类型：
--
作者：
Banerjee, Saswati;Dhara, Sujoy K.;Bacanamwo, Methode
内皮细胞（EC）在发育过程中的血管发生和器官发生以及癌症和心血管疾病的发病机制中是重要的。然而，很少有EC规格因素是已知的，初级EC生产仍然效率低下。基于最近的研究暗示内皮糖蛋白（Eng）在早期血管发育和血管生成，我们假设，Eng可能是EC特化基因。用重组Eng或表达Eng ORF的质粒处理小鼠胚胎干细胞（ESC），并在存在或不存在骨形态发生蛋白4（BMP 4）的情况下分化。中胚层和EC标记基因的表达，EC规范和其下游目标的已知介质进行了监测，通过定量PCR，蛋白质印迹，免疫细胞化学，流式细胞术。通过体外血管生成试验和TNF-α处理诱导Icam 1表达来评估分化的EC的功能。重组Eng和强制Eng表达增加了表达EC标志基因VE-钙粘蛋白、vWF和Tie 2的功能性EC的数量，并增强了BMP 4的作用。Eng-induced EC分化独立于EC特异性的已知介质，如Indian Hedgehog（IHH）和BMP 4或BMP 4/Smad 1/5/8信号传导。这些研究表明，Eng是一个新的EC特化基因。
Endothelial cells (EC) are important in vasculogenesis and organogenesis during development and in the pathogenesis of cancer and cardiovascular diseases. However, few EC specification factors are known and primary EC production remains inefficient. Based on recent studies implicating endoglin (Eng) in early vascular development and angiogenesis, we hypothesized that Eng may be an EC specification gene. Mouse embryonic stem cells (ESC) were treated with recombinant Eng or a plasmid expressing the Eng ORF, and differentiated in the presence or absence of bone morphogenic protein 4 (BMP4). Expression of the mesoderm and EC marker genes, the known mediators of EC specification and their downstream targets was monitored by quantitative PCR, western blot, immunocytochemistry, and flow cytometry. Functionality of the differentiated EC was assessed by in vitro angiogenesis assay and the induction of Icam1 expression in response to TNF-α treatment. Both recombinant Eng and forced Eng expression increased the number of functional EC expressing the EC marker genes VE-cadherin, vWF, and Tie2, and enhanced the effect of BMP4. The Eng-induced EC differentiation was independent of known mediators of EC specification such as Indian Hedgehog (IHH) and BMP4 or of BMP4/Smad1/5/8 signaling. These studies suggest that Eng is a novel EC specification gene.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
3.7
作者：
Banerjee S;Bacanamwo M
通讯作者：
Bacanamwo M
影响因子：
30.8
作者：
Liu, PT;Wakamiya, M;Bradley, A
通讯作者：
Bradley, A
影响因子：
4.6
作者：
Pearson, Stella;Sroczynska, Patrycja;Kouskoff, Valerie
通讯作者：
Kouskoff, Valerie
DOI：
10.1161/atvbaha.109.184200
发表时间：
2009-05
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Kelly MA;Hirschi KK
通讯作者：
Hirschi KK