Comparative analysis of differentially abundant proteins between high and low intramuscular fat content groups in donkeys.
Comparative analysis of differentially abundant proteins between high and low intramuscular fat content groups in donkeys.
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DOI：
10.3389/fvets.2022.951168
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发表时间：
2022
影响因子：
3.2
通讯作者：
Zhang, Shuyi
中科院分区：
文献类型：
--
作者：
Tan, Xiaofan;He, Yu;Qin, Yanchun;Yan, Zhiwei;Chen, Jing;Zhao, Ruixue;Zhou, Shenglan;Irwin, David M.;Li, Bojiang;Zhang, Shuyi
肌内脂肪（IMF）是决定肉品质的重要调节剂，其含量与肉的风味、嫩度和多汁性密切相关。许多研究已经使用定量蛋白质组学分析来鉴定与牲畜肉质性状相关的蛋白质，然而，影响驴肌肉IMF的潜在候选蛋白质尚未完全了解。在这项研究中，我们对驴背最长肌（LD）样本进行了定量蛋白质组学分析，采用串联质量标记（TMT）标记。从本研究中使用的6个肌肉样本中共鉴定出585,555个光谱。总共检测到20,583条肽，其中包括15,279条独特肽，鉴定了2,540种蛋白质。我们分析了高(H)和低(L) IMF含量的驴LD肌肉中蛋白质（DAPs）的差异。我们在H和L IMF含量组之间鉴定了30个DAPs，其中17个在H IMF含量组上调，13个下调。基因本体（GO）和京都基因与基因组百科全书（KEGG）功能富集分析揭示了许多参与脂质代谢和脂肪形成的GO术语（如骨形态发生蛋白（BMP）受体结合）和途径（如Wnt信号通路和Hippo信号通路）。蛋白质-蛋白质相互作用网络的构建鉴定了参与这些网络的16个dap。我们的数据为未来研究参与IMF沉积的候选蛋白质和改善驴肉质的潜在新方法提供了基础。
Intramuscular fat (IMF) is an important regulator that determines meat quality, and its content is closely related to flavor, tenderness, and juiciness. Many studies have used quantitative proteomic analysis to identify proteins associated with meat quality traits in livestock, however, the potential candidate proteins that influence IMF in donkey muscle are not fully understood. In this study, we performed quantitative proteomic analysis, with tandem-mass-tagged (TMT) labeling, with samples from the longissimus dorsi (LD) muscle of the donkey. A total of 585,555 spectra were identified from the six muscle samples used in this study. In total, 20,583 peptides were detected, including 15,279 unique peptides, and 2,540 proteins were identified. We analyzed differentially abundant proteins (DAPs) between LD muscles of donkeys with high (H) and low (L) IMF content. We identified 30 DAPs between the H and L IMF content groups, of which 17 were upregulated and 13 downregulated in the H IMF group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis of these DAPs revealed many GO terms (e.g., bone morphogenetic protein (BMP) receptor binding) and pathways (e.g., Wnt signaling pathway and Hippo signaling pathway) involved in lipid metabolism and adipogenesis. The construction of protein–protein interaction networks identified 16 DAPs involved in these networks. Our data provide a basis for future investigations into candidate proteins involved in IMF deposition and potential new approaches to improve meat quality in the donkey.
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影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
影响因子：
3.7
作者：
Li B;Feng C;Zhu S;Zhang J;Irwin DM;Zhang X;Wang Z;Zhang S
通讯作者：
Zhang S
影响因子：
8.8
作者：
Hou, Xinhua;Liu, Qiufeng;Wang, Lixian
通讯作者：
Wang, Lixian
影响因子：
1.7
作者：
De Palo, Pasquale;Maggiolino, Aristide;Tateo, Alessandra
通讯作者：
Tateo, Alessandra
影响因子：
--
作者：
Listrat A;Lebret B;Louveau I;Astruc T;Bonnet M;Lefaucheur L;Picard B;Bugeon J
通讯作者：
Bugeon J