High expression of TACC3 in esophageal squamous cell carcinoma correlates with poor prognosis.
High expression of TACC3 in esophageal squamous cell carcinoma correlates with poor prognosis.
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TACC3在食管鳞状细胞癌中的高表达与不良预后相关
DOI：
10.18632/oncotarget.3190
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发表时间：
2015-03-30
期刊：
影响因子：
--
通讯作者：
Wen ZS
中科院分区：
文献类型：
--
作者：
Huang ZL;Lin ZR;Xiao YR;Cao X;Zhu LC;Zeng MS;Zhong Q;Wen ZS
为分析转化酸性卷曲螺旋蛋白3（TACC 3）在食管鳞状细胞癌（ESCC）组织中的表达，探讨TACC 3能否作为ESCC诊断和预后的生物标志物。此外，检测TACC 3基因敲低的ESCC细胞的细胞生长、集落形成、迁移能力和上皮-间质转化标志物。与非肿瘤性食管上皮组织相比，TACC 3的mRNA和蛋白水平在ESCC标本中更高。IHC结果显示TACC 3表达与分化（p = 0.017）和淋巴结状态（p = 0.028）显著相关。TACC 3高表达患者的预后显著差于低表达患者（p = 0.017），尤其是在I-II期患者中（p = 0.028）。多因素分析表明TACC 3表达是ESCC患者的独立预后因素（p = 0.025）。TACC 3的敲低抑制了细胞的增殖、集落形成和迁移能力。本研究首次发现TACC 3不仅可以作为诊断和预后ESCC的生物标志物，而且可以作为ESCC患者的潜在治疗靶点。
To analyze the expression of the transforming acidic coiled-coil protein 3 (TACC3) in esophageal squamous cell carcinoma (ESCC) samples, and to identify whether TACC3 can serve as a biomarker for the diagnosis and prognosis of ESCC, qPCR, western blotting and immunohistochemistry staining (IHC) were utilized to detect the expression of TACC3. Furthermore, cell growth, colony formation, migration ability and the epithelial-mesenchymal transition markers of ESCC cells in which TACC3 were knocked-down were measured. The mRNA and protein levels of TACC3 were higher in ESCC specimens compared to non-tumorous esophageal epithelial tissues. IHC results revealed TACC3 expression was significantly correlated to differentiation (p = 0.017) and lymphoid nodal status (p = 0.028). The patients with high-expression of TACC3 had a significantly poor prognosis compared to those of low-expression (p = 0.017), especially in the patients at stages I–II (p = 0.028). Multivariate analysis indicated that TACC3 expression was an independent prognostic factor for ESCC patients (p = 0.025). Knockdown of TACC3 inhibited the ability of cell proliferation, colony formation and migration. This study first identifies TACC3 not only as a useful biomarker for diagnose and prognosis of ESCC, but also as a potential therapeutic target for patients with ESCC.
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影响因子：
30.8
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