Tylosin Inhibits Streptococcus suis Biofilm Formation by Interacting With the O-acetylserine (thiol)-lyase B CysM.
Tylosin Inhibits Streptococcus suis Biofilm Formation by Interacting With the O-acetylserine (thiol)-lyase B CysM.
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DOI：
10.3389/fvets.2021.829899
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发表时间：
2021
影响因子：
3.2
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Zhou Y;Yu F;Chen M;Zhang Y;Qu Q;Wei Y;Xie C;Wu T;Liu Y;Zhang Z;Chen X;Dong C;Che R;Li Y
猪链球菌（S. suis）可以通过生物膜的形成降低其毒力或改变局部条件，从而促进体内感染的持续存在。生物膜的形成是慢性耐药猪链球菌感染的重要原因。本研究的目的是评估泰络素是否通过与o -乙酰丝氨酸（硫醇）裂解酶B （CysM）相互作用有效抑制猪链球菌生物膜的形成，CysM是半胱氨酸合成的关键酶调节因子。与野生型ATCC 700794菌株相比，突变株（ΔcysM）的生物膜形成显著降低。Tylosin抑制cysM基因表达，降低细胞外基质含量，降低半胱氨酸、同型半胱氨酸和s -腺苷蛋氨酸水平，表明其作为猪链球菌生物膜形成有效抑制剂的潜在价值。此外，利用生物层干涉技术和傅里叶变换红外光谱，我们发现tylosin和CysM可以直接结合。总之，这些结果提供了泰络素通过与CysM相互作用抑制猪链球菌生物膜形成的证据。
Streptococcus suis (S. suis) can decrease its virulence or modify local conditions through biofilm formation, which promotes infection persistence in vivo. Biofilm formation is an important cause of chronic drug-resistant S. suis infection. The aim of this study was to evaluate whether tylosin effectively inhibits S. suis biofilm formation by interacting with O-acetylserine (thiol)-lyase B (CysM), a key enzymatic regulator of cysteine synthesis. Biofilm formation of the mutant (ΔcysM) strain was significantly lower compared to the wild-type ATCC 700794 strain. Tylosin inhibited cysM gene expression, decreased extracellular matrix contents, and reduced cysteine, homocysteine, and S-adenosylmethionine levels, indicating its potential value as an effective inhibitor of S. suis biofilm formation. Furthermore, using biolayer interferometry technology and fourier-transform infrared spectroscopy, we found that tylosin and CysM could be combined directly. Overall, these results provide evidence that tylosin inhibits S. suis biofilm formation by interacting with CysM.
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影响因子：
1.2
作者：
Desai, Malhar;Di, Rong;Fan, Huizhou
通讯作者：
Fan, Huizhou
影响因子：
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作者：
Akers KS;Mende K;Cheatle KA;Zera WC;Yu X;Beckius ML;Aggarwal D;Li P;Sanchez CJ;Wenke JC;Weintrob AC;Tribble DR;Murray CK;Infectious Disease Clinical Research Program Trauma Infectious Disease Outcomes Study Group
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Infectious Disease Clinical Research Program Trauma Infectious Disease Outcomes Study Group
影响因子：
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作者：
Rathsam, C;Eaton, RE;Jacques, NA
通讯作者：
Jacques, NA
影响因子：
3.6
作者：
Soutourina, Olga;Poupel, Olivier;Martin-Verstraete, Isabelle
通讯作者：
Martin-Verstraete, Isabelle
影响因子：
2.9
作者：
Claus, MT;Zocher, GE;Schulz, GE
通讯作者：
Schulz, GE