Taurine protects hamster bronchioles from acute NO2-induced alterations. A histologic, ultrastructural, and freeze-fracture study.
Taurine protects hamster bronchioles from acute NO2-induced alterations. A histologic, ultrastructural, and freeze-fracture study.
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牛磺酸可保护仓鼠细支气管免受 NO2 引起的急性改变。
DOI：
--
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发表时间：
1986
影响因子：
6
通讯作者：
D. Solano
中科院分区：
文献类型：
--
作者：
R. Gordon;A. Shaked;D. Solano
在这项研究中，作者描述了使用饮食牛磺酸保护仓鼠肺上皮免受急性二氧化氮（NO2）损伤。结论是基于组织学，超微结构和冷冻断裂分析。仓鼠预处理14天，0.5%的牛磺酸在他们的饮用水。然后将它们暴露在7或30 ppm的NO2中24小时。将这些实验组动物的肺与仅用NO2处理的仓鼠的肺、仅给予牛磺酸的仓鼠的肺和未处理的对照组的肺进行比较。处理后，将仓鼠麻醉，并使用含有1%戊二醛、4%多聚甲醛和0.2 M二甲胂酸盐的溶液通过心脏右侧灌注固定。将气管和肺整块取出并在4 ℃下于二甲胂酸盐缓冲液中保存过夜.从每个肺叶解剖终末和呼吸性细支气管，包括肺泡管和细支气管周围肺泡，并进行Epon包埋和冷冻断裂复制。光镜和透射电镜显示，典型的炎症细胞浸润在细支气管和肺泡管区域在仓鼠暴露于NO2的肺。细支气管上皮扁平，纤毛细胞纤毛脱落，Clara细胞顶端突起。克拉拉细胞分泌颗粒减少或消失。冷冻断裂复制品的细支气管上皮细胞紧密连接的形态学技术分析表明，减少和断裂的原纤维。只有动物暴露于30 ppm的NO2表现出生理细胞间的辣根过氧化物酶的渗透。仓鼠预处理牛磺酸，然后暴露于NO2没有显示这些变化。它们表现出与未处理对照组和仅用牛磺酸处理的仓鼠相同的形态学特征。在此基础上，提示预防性饮食牛磺酸可预防急性NO2诱导的形态学肺损伤。牛磺酸也可以有效预防由其他氧化剂气体引起的肺损伤。
In this study the authors describe the use of dietary taurine to protect hamster lung epithelium from acute nitrogen dioxide (NO2) injury. The conclusions were based on histologic, ultrastructural, and freeze-fracture analyses. Hamsters were pretreated for 14 days with 0.5% taurine in their drinking water. They were then exposed to either 7 or 30 ppm NO2 for 24 hours. The lungs from animals of these experimental groups were compared with those from hamsters treated with only NO2, and those given only taurine and with untreated controls. After treatment, hamsters were anesthetized and perfusion-fixed through the right side of the heart with a solution containing 1% glutaraldehyde, 4% paraformaldehyde, and 0.2 M cacodylate. The trachea and lungs were removed en bloc and stored overnight in cacodylate buffer at 4 C. Terminal and respiratory bronchioles, including alveolar ducts and peribronchiolar alveoli, were dissected from each lobe and processed for embedding in Epon and freeze-fracture replication. Light and transmission electron microscopy revealed the typical inflammatory cell infiltrate in the bronchiolar and alveolar duct regions in the lungs of hamsters exposed to NO2. The bronchiolar epithelium appeared flattened because of loss and breakage of cilia on ciliated cells and apical protrusions of Clara cells. Clara-cell secretory granules were reduced or absent. Freeze-fracture replicas of tight junctions of bronchiolar epithelium analyzed by morphometric techniques demonstrated a reduction and fragmentation of fibrils. Only animals exposed to 30 ppm NO2 exhibited physiologic intercellular penetration of horseradish peroxidase. Hamsters pretreated with taurine and then exposed to NO2 showed none of these alterations. They exhibited the same morphologic features as the untreated controls and the hamsters treated only with taurine. On the basis of this evidence, it is suggested that prophylactic dietary taurine can prevent acute NO2-induced morphologic lung injury. Taurine may also be effective in preventing lung injury induced by other oxidant gases.
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影响因子：
9.6
作者：
Evans,MJ;Cabral-Anderson,LJ;Dekker,NP;Freeman,G
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DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Thomas,EL;Grisham,MB;Melton,DF;Jefferson,MM
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影响因子：
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作者：
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Kleinerman,J
体外吞噬中性粒细胞产生的上皮通透性。
DOI：
--
发表时间：
1986
期刊：
The American review of respiratory disease
影响因子：
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作者：
Sugahara,K;Cott,GR;Parsons,PE;Mason,RJ;Sandhaus,RA;Henson,PM
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Henson,PM
自由基病理学：二氧化氮与过氧化氢反应副产物人α-1-蛋白酶抑制剂的失活以及肺气肿的病因学。
DOI：
10.1016/0006-291x(82)91807-1
发表时间：
1982
影响因子：
3.1
作者：
Dooley,MM;Pryor,WA
通讯作者：
Pryor,WA