Cell Adhesion on Micro-Structured Fibronectin Gradients Fabricated by Multiphoton Excited Photochemistry.
Cell Adhesion on Micro-Structured Fibronectin Gradients Fabricated by Multiphoton Excited Photochemistry.
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DOI：
10.1007/s12195-012-0237-8
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发表时间：
2012-09
影响因子：
2.8
通讯作者：
Campagnola, Paul J.
中科院分区：
文献类型：
--
作者：
Chen, Xiyi;Su, Yuan-Deng;Ajeti, Visar;Chen, Shean-Jen;Campagnola, Paul J.
ECM蛋白的浓度梯度在细胞生物学的许多领域，包括伤口愈合和转移中起着积极的作用。它们也可以形成组织工程支架的基础，因为它们可以指导细胞粘附和迁移，并促进新的基质合成。为了更好地了解吸引梯度上的细胞与基质相互作用，我们使用多光子激发（MPE）光化学方法从纤连蛋白（FN）中制造了共价连接的微结构梯度。梯度设计由一系列平行的单个线性梯度组成，总尺寸约为800 × 800 μm，其中浓度的线性动态范围接近10倍。研究了3 T3成纤维细胞的粘附动力学，其中细胞形态和肌动蛋白细胞骨架变得越来越长，并且在增加的蛋白质浓度下与梯度的方向对齐。此外，当粘附到不同FN浓度但具有相似形貌的区域时，细胞形态是不同的。这些结果表明，制造方法允许调查的接触指导和ECM线索的细胞-基质相互作用的作用。我们认为这种设计克服了其他制造方法的一些限制，特别是在3D图案化能力方面，并将作为研究细胞-基质相互作用的新工具。
Concentration gradients of ECM proteins play active roles in many areas of cell biology including wound healing and metastasis. They may also form the basis of tissue engineering scaffolds, as these can direct cell adhesion and migration and promote new matrix synthesis. To better understand cell–matrix interactions on attractive gradients, we have used multiphoton excited (MPE) photochemistry to fabricate covalently linked micro-structured gradients from fibronectin (FN). The gradient design is comprised of a parallel series of individual linear gradients with overall dimensions of approximately 800 × 800 μm, where a linear dynamic range of nearly 10-fold in concentration was achieved. The adhesion dynamics of 3T3 fibroblasts were investigated, where the cell morphology and actin cytoskeleton became increasingly elongated and aligned with the direction of the gradient at increasing protein concentration. Moreover, the cell morphologies are distinct when adhered to regions of differing FN concentration but with similar topography. These results show that the fabrication approach allows investigating the roles of contact guidance and ECM cues on the cell–matrix interactions. We suggest this design overcomes some of the limitations with other fabrication methods, especially in terms of 3D patterning capabilities, and will serve as a new tool to study cell–matrix interactions.
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影响因子：
5.3
作者：
Mai, Junyu;Fok, Lee;Poo, Mu-ming
通讯作者：
Poo, Mu-ming
影响因子：
3.9
作者：
Gunawan, RC;Silvestre, J;Leckband, DE
通讯作者：
Leckband, DE
DOI：
10.1083/jcb.200810041
发表时间：
2009-02-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Doyle AD;Wang FW;Matsumoto K;Yamada KM
通讯作者：
Yamada KM
影响因子：
2.7
作者：
BRANDLEY, BK;SCHNAAR, RL
通讯作者：
SCHNAAR, RL
影响因子：
14
作者：
Fricke, Rita;Zentis, Peter D.;Meffert, Simone H.
通讯作者：
Meffert, Simone H.