The extracellular release of DNA and HMGB1 from Jurkat T cells during in vitro necrotic cell death.
The extracellular release of DNA and HMGB1 from Jurkat T cells during in vitro necrotic cell death.
复制标题
DOI：
10.1177/1753425912437981
复制
发表时间：
2012-10
期刊：
影响因子：
3.2
通讯作者：
Pisetsky DS
中科院分区：
文献类型：
--
作者：
Beyer C;Stearns NA;Giessl A;Distler JH;Schett G;Pisetsky DS
在先天免疫中，死亡和濒临死亡的细胞释放内部成分，这些成分可以作为损伤相关的分子模式或警报。这种释放在坏死过程中比在凋亡过程中发生得更多，这可能解释了这些死亡形式的免疫学特性的差异。为了阐明坏死中的潮湿释放，我们比较了冻融、乙醇、热或过氧化氢处理Jurkat T细胞后，两种核分子(DNA和HMGB1，一种具有Alarmin活性的非组蛋白蛋白)在介质中的水平。在我们的实验中，DNA的释放是用荧光染料PicoGreen来测量的，而HMGB1的释放是用Western blotting来测量的。这项研究的结果表明，每种形式的坏死都与DNA和HMGB1的释放在动力学和数量上有不同的模式。其中，冻融产生的HMGB1和DNA水平的增加最高和最快，尽管释放的DNA受到核酸酶的消化；此外，冻融导致了通过流式细胞仪测量的颗粒的产生。综上所述，这些结果表明，实验性坏死导致不同的核分子释放模式，这可能会影响它们的免疫活性。
In innate immunity, dead and dying cells release internal constituents that can serve as DAMPs (damage-associated molecular patterns) or alarmins. This release occurs more abundantly during necrosis than apoptosis and may account for the differences in the immunological properties of these death forms. To elucidate DAMP release in necrosis, we have compared the levels of two nuclear molecules (DNA and HMGB1, a non-histone protein with alarmin activity) in the media following necrosis of Jurkat T cells by freeze-thawing, ethanol, heat or hydrogen peroxide. In our experiments, DNA release was measured by fluorimetry with the dye PicoGreen, while HMGB1 was measured by Western blotting. As results of this study show, each form of necrosis is associated with a distinct pattern of DNA and HMGB1 release with respect to kinetics and amounts. Of these, freeze-thawing produced the highest and most rapid increase in HMGB1 and DNA levels although the released DNA was subject to nuclease digestion; in addition, freeze-thawing led to the production of particles measured by flow cytometry. Together, these results indicate that experimental necrosis leads to diverse patterns of nuclear molecule release which could affect their immunological activity.
登录
查看更多内容
影响因子：
5.7
作者：
Antoine, Daniel James;Williams, Dominic P.;Park, B. Kevin
通讯作者：
Park, B. Kevin
影响因子：
3
作者：
Ahmad, Fateh;Gravante, Gianpiero;Lloyd, David M.
通讯作者：
Lloyd, David M.
影响因子：
82.9
作者：
Chen, Chun-Jen;Kono, Hajime;Rock, Kenneth L.
通讯作者：
Rock, Kenneth L.
影响因子：
14.8
作者：
Degterev, Alexei;Hitomi, Junichi;Yuan, Junying
通讯作者：
Yuan, Junying
影响因子：
5.6
作者：
Kim, Cho Hee;Han, Song Iy;Kang, Ho Sung
通讯作者：
Kang, Ho Sung