Characterization of the N-terminal tail domain of histone H3 in condensed nucleosome arrays by hydrogen exchange and NMR.
Characterization of the N-terminal tail domain of histone H3 in condensed nucleosome arrays by hydrogen exchange and NMR.
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DOI：
10.1021/ja9070078
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发表时间：
2009-10-28
影响因子：
15
通讯作者：
Bai, Yawen
中科院分区：
文献类型：
--
作者：
Kato, Hidenori;Gruschus, James;Ghirlando, Rodolfo;Tjandra, Nico;Bai, Yawen
组蛋白的N端尾结构域（NTDs）在染色质高级结构的形成和基因功能的调控中起着重要作用。虽然核小体核心颗粒的结构已经通过X射线晶体学在近原子分辨率下确定，但在该结构中没有观察到组蛋白尾部。在这里，我们证明了大量的核小体阵列与明确的DNA定位可以重建使用特定的DNA序列和重组同位素标记的组蛋白，允许通过酰胺氢交换和多维核磁共振（NMR）方法的NTD构象的调查。我们研究了果蝇组蛋白H3在凝聚核小体阵列的NTD。结果表明，H3的NTD中的大多数酰胺质子被保护免于交换，这与NTD形成折叠结构一致。我们的研究表明，氢交换结合NMR可以提供组蛋白NTD在浓缩核小体阵列中的残基表征，该技术可用于研究其他组蛋白和具有翻译后修饰的组蛋白的NTD。
The N-terminal tail domains (NTDs) of histones play important roles in the formation of higher-order structures of chromatin and the regulation of gene functions. Although the structure of the nucleosome core particle has been determined by X-ray crystallography at near-atomic resolution, the histone tails are not observed in this structure. Here, we demonstrate that large quantities of nucleosome arrays with well-defined DNA positioning can be reconstituted using specific DNA sequences and recombinant isotope-labeled histones, allowing for the investigation of NTD conformations by amide hydrogen exchange and multi-dimensional nuclear magnetic resonance (NMR) methods. We examined the NTD of Drosophila melanogaster histones H3 in condensed nucleosome arrays. The results reveal that the majority of the amide protons in the NTD of H3 are protected from exchange, consistent with the NTDs having formed folded structures. Our study demonstrates hydrogen exchange coupled with NMR can provide residue-by-residue characterization of NTDs of histones in condensed nucleosome arrays, a technique that may be used to study NTDs of other histones and those with post-translational modifications.
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DOI：
10.1002/prot.340170110
发表时间：
1993-09-01
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
BAI, YW;MILNE, JS;ENGLANDER, SW
通讯作者：
ENGLANDER, SW
DOI：
10.1038/nsb792
发表时间：
2002-05-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Hoshino, M;Katou, H;Goto, Y
通讯作者：
Goto, Y
影响因子：
64.8
作者：
Luger, K;Mader, AW;Richmond, TJ
通讯作者：
Richmond, TJ
影响因子：
64.8
作者：
Schalch, T;Duda, S;Richmond, TJ
通讯作者：
Richmond, TJ
影响因子：
5.6
作者：
Dorigo, B;Schalch, T;Richmond, TJ
通讯作者：
Richmond, TJ