Mycobacterium tuberculosis infection is associated with the development of erythema nodosum and nodular vasculitis.
Mycobacterium tuberculosis infection is associated with the development of erythema nodosum and nodular vasculitis.
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结核分枝杆菌感染与结节性红斑和结节性血管炎的发生有关
DOI：
10.1371/journal.pone.0062653
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Chen S;Chen J;Chen L;Zhang Q;Luo X;Zhang W
背景结核分枝杆菌（MTB）感染被认为是结节性红斑（EN）和结节性血管炎（NV）的发病机制之一，这两种疾病都是典型的血管炎。然而，几乎没有证据表明MTB存在于皮肤病变中。本研究旨在评估中国人群中MTB感染与EN和NV发展之间的关系。方法选取血管炎患者107例（EN 36例，NV 27例，other 44例）和其他皮肤病患者40例作为对照，采用实时聚合酶链反应（PCR）对MTB的IS6110和mpt64基因片段进行分析。通过QuantiFERON®-TB金管（IT）检测他们的血液单个核细胞对MTB抗原特异性IFN-γ的反应。结果PCR检测结果显示，EN和NV标本中IS6110基因阳性的比例分别为7/23（30.4%）和7/18(38.9%)，显著高于其他血管炎（OV）标本中3/34（8.8%）和对照标本中3/40 (7.5%)（p<0.05）。巢式Real-Time PCR检测结果显示，6/7（86%）的is6110阳性的EN样本、所有is6110阳性的NV和对照样本中mpt64基因阳性，而is6110阳性的OV样本中只有1/3的mpt64基因阳性。EN组19/32（59.4%）、NV组20/26（76.9%）、OV组17/36 (47.2%)MTB抗原特异性IFN-γ阳性，均显著高于对照组6/40 (15%)（p<0.05）。结论MTB感染和活动性TB与中国人NV和EN的发生有关。
Background Mycobacterium tuberculosis (MTB) infection has been suggested to contribute to the pathogenesis of erythema nodosum (EN) and nodular vasculitis (NV), the classic forms of panniculitis. However, there is little evidence to demonstrate the presence of MTB in the skin lesions. This study is aimed at evaluating the association between MTB infection and the development of EN and NV in a Chinese population. Methods A total of 107 patients (36 EN, 27 NV, and 44 others) with vasculitis and 40 control cases with other skin diseases were recruited and their skin lesion samples were subjected to real time polymerase chain reaction (PCR) analysis of the IS6110 and mpt64 gene fragments of MTB. Their blood mononuclear cells were tested for MTB antigen-specific IFN-γ responses by QuantiFERON®-TB Gold In-Tube (IT) assays. Results PCR analysis revealed that 7/23 (30.4%) and 7/18 (38.9%) of the EN and NV samples were positive for the IS6110 DNA, respectively, which were significantly higher than 3/34 (8.8%) of other vasculitis (OV) and 3/40 (7.5%) of the control samples (p<0.05). The nested Real-Time PCR assay indicated that 6/7 (86%) of the IS6110-positive EN samples, all of the IS6110-positive NV and control samples, but only 1/3 of the IS6110-positive OV samples, were positive for the mpt64 gene. Similarly, 19/32 (59.4%) of the EN patients, 20/26 (76.9%) of the NV patients, and 17/36 (47.2%) of the OV patients were positive for MTB antigen-specific IFN-γ responses, which were significantly higher than 6/40 (15%) of the controls (p<0.05). Conclusion Our data strongly suggest that MTB infection and active TB are associated with the development of NV and EN in Chinese.
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影响因子：
3.7
作者：
Feng Y;Diao N;Shao L;Wu J;Zhang S;Jin J;Wang F;Weng X;Zhang Y;Zhang W
通讯作者：
Zhang W
影响因子：
3.6
作者：
Gilchrist, Heidi;Patterson, James W.
通讯作者：
Patterson, James W.
影响因子：
2.8
作者：
El Khechine, Amel;Henry, Mireille;Drancourt, Michel
通讯作者：
Drancourt, Michel
影响因子：
--
作者：
Baselga, E;Margall, N;deMoragas, J
通讯作者：
deMoragas, J
影响因子：
3.6
作者：
Gupta, SN;Flaherty, JP;Shaw, JC
通讯作者：
Shaw, JC