Surface acoustic wave nebulization facilitating lipid mass spectrometric analysis.
Surface acoustic wave nebulization facilitating lipid mass spectrometric analysis.
复制标题
DOI：
10.1021/ac300807p
复制
发表时间：
2012-08-07
影响因子：
7.4
通讯作者：
Goodlett DR
中科院分区：
文献类型：
--
作者：
Yoon SH;Huang Y;Edgar JS;Ting YS;Heron SR;Kao Y;Li Y;Masselon CD;Ernst RK;Goodlett DR
Surface acoustic wave nebulization (SAWN) is a novel method to transfer non-volatile analytes directly from the aqueous-phase to the gas-phase for mass spectrometric analysis. The lower ion energetics of SAWN and its planar nature make it appealing for analytically challenging lipid samples. This challenge is a result of their amphipathic nature, labile nature, and tendency to form aggregates, which readily precipitate clogging capillaries used for electrospray ionization (ESI). Here we report the use of SAWN to characterize the complex glycolipid, lipid A, which serves as the membrane anchor component of lipopolysaccharide (LPS) and has a pronounced tendency to clog nano-ESI capillaries. We also show that unlike ESI SAWN is capable of ionizing labile phospholipids without fragmentation. Lastly, we compare the ease of use of SAWN to the more conventional infusion-based ESI methods and demonstrate the ability to generate higher order tandem mass spectral data of lipid A for automated structure assignment using our previously reported hierarchical tandem mass spectrometry (HiTMS) algorithm. The ease of generating SAWN-MSn data combined with HiTMS interpretation offers the potential for high throughput lipid A structure analysis.
登录
查看更多内容
影响因子：
3.4
作者：
Coats SR;Jones JW;Do CT;Braham PH;Bainbridge BW;To TT;Goodlett DR;Ernst RK;Darveau RP
通讯作者：
Darveau RP
影响因子：
4.4
作者：
Eng, Jimmy K.;Fischer, Bernd;MacCoss, Michael J.
通讯作者：
MacCoss, Michael J.
影响因子：
7.4
作者：
Ekroos, K;Chernushevich, IV;Shevchenko, A
通讯作者：
Shevchenko, A
影响因子：
3.5
作者：
Alvarez, Mar;Friend, James;Yeo, Leslie Y.
通讯作者：
Yeo, Leslie Y.
影响因子：
3.6
作者：
Kannenberg, EL;Carlson, RW
通讯作者：
Carlson, RW