Mouse lung mechanical properties under varying inflation volumes and cycling frequencies.
Mouse lung mechanical properties under varying inflation volumes and cycling frequencies.
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DOI：
10.1038/s41598-022-10417-3
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发表时间：
2022-05-02
影响因子：
4.6
通讯作者：
Eskandari, M.
中科院分区：
文献类型：
--
作者：
Quiros, K. A. M.;Nelson, T. M.;Sattari, S.;Mariano, C. A.;Ulu, A.;Dominguez, E. C.;Nordgren, T. M.;Eskandari, M.
呼吸道病变改变肺的结构并影响其力学。小鼠被广泛用于肺部病理学的研究，但缺乏基本的机械测量评估不同的充气体积和循环频率的相互依赖的影响。在这项研究中，5只雄性C57 BL/6 J小鼠（29-33周龄）肺的机械性能进行了评价离体使用我们定制设计的机电，连续测量通气装置。我们全面量化和分析了负荷量（0.3，0.5，0.7，0.9 ml）和呼吸频率（5，10，20次呼吸/min）对肺充气和放气力学性能的影响。我们报告了静态顺应性平均值为5.4-16.1 µl/cmH 2 O，放气顺应性平均值为5.3-22.2 µl/cmH 2 O，松弛百分比平均值为21.7- 39.1%，滞后平均值为1.11-7.6 ml·cmH 2 O，能量损失平均值为39-58%，测试范围为四种体积和三种速率，沿着其他测量。我们的结论是，充气量被发现显着影响滞后，静态顺应性，开始顺应性，顶部顺应性，放气顺应性，和百分之松弛，和循环速率被发现只影响滞后，能量损失，百分之松弛，静态顺应性和放气顺应性。
Respiratory pathologies alter the structure of the lung and impact its mechanics. Mice are widely used in the study of lung pathologies, but there is a lack of fundamental mechanical measurements assessing the interdependent effect of varying inflation volumes and cycling frequency. In this study, the mechanical properties of five male C57BL/6J mice (29–33 weeks of age) lungs were evaluated ex vivo using our custom-designed electromechanical, continuous measure ventilation apparatus. We comprehensively quantify and analyze the effect of loading volumes (0.3, 0.5, 0.7, 0.9 ml) and breathing rates (5, 10, 20 breaths per minute) on pulmonary inflation and deflation mechanical properties. We report means of static compliance between 5.4–16.1 µl/cmH2O, deflation compliance of 5.3–22.2 µl/cmH2O, percent relaxation of 21.7–39.1%, hysteresis of 1.11–7.6 ml•cmH2O, and energy loss of 39–58% for the range of four volumes and three rates tested, along with additional measures. We conclude that inflation volume was found to significantly affect hysteresis, static compliance, starting compliance, top compliance, deflation compliance, and percent relaxation, and cycling rate was found to affect only hysteresis, energy loss, percent relaxation, static compliance and deflation compliance.
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影响因子：
3.3
作者：
HILDEBRANDT, J
通讯作者：
HILDEBRANDT, J
影响因子：
3.3
作者：
Eskandari, Mona;Arvayo, Alberto L.;Levenston, Marc E.
通讯作者：
Levenston, Marc E.
影响因子：
9.6
作者：
Gelb, AF;Licuanan, J;Zamel, N
通讯作者：
Zamel, N
影响因子：
3.3
作者：
Hickling, Keith G
通讯作者：
Hickling, Keith G
DOI：
10.1016/j.jmbbm.2019.02.024
发表时间：
2019-06-01
影响因子：
3.9
作者：
Birzle, Anna M.;Wall, Wolfgang A.
通讯作者：
Wall, Wolfgang A.