Piezo1 ion channel pore properties are dictated by C-terminal region.
Piezo1 ion channel pore properties are dictated by C-terminal region.
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DOI：
10.1038/ncomms8223
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发表时间：
2015-05-26
影响因子：
16.6
通讯作者：
Patapoutian, Ardem
中科院分区：
文献类型：
--
作者：
Coste, Bertrand;Murthy, Swetha E.;Mathur, Jayanti;Schmidt, Manuela;Mechioukhi, Yasmine;Delmas, Patrick;Patapoutian, Ardem
Piezo 1和Piezo 2编码机械激活的阳离子通道，分别作为机械换能器参与血管系统发育和触摸感应。Piezos的结构特征仍然未知。生物信息学预测小鼠Piezo 1具有30-40个跨膜（TM）结构域。在这里，我们发现，9个假定的跨膜间区域可从细胞外侧。我们使用mPiezo 1和dPiezo之间的嵌合体来表明离子渗透性能由C-末端区域赋予。我们进一步确定了一个谷氨酸残基内的保守区域相邻的最后两个推定的TM结构域的蛋白质，突变时，影响单一的电导和离子选择性，并调制孔块。我们建议，这种氨基酸要么是在孔或密切相关的孔。我们的研究结果描述了这个通道家族的重要结构基序，并为压电晶体如何机械门控和传导离子的机械理解奠定了基础。 压电离子通道作为机械传感器参与血管发育和触觉感知，但其结构特征仍然未知。在这里，作者发现压电蛋白的C-末端区域包含孔，并在该区域内确定了参与离子传导特性的谷氨酸残基。
Piezo1 and Piezo2 encode mechanically activated cation channels that function as mechanotransducers involved in vascular system development and touch sensing, respectively. Structural features of Piezos remain unknown. Mouse Piezo1 is bioinformatically predicted to have 30–40 transmembrane (TM) domains. Here, we find that nine of the putative inter-transmembrane regions are accessible from the extracellular side. We use chimeras between mPiezo1 and dPiezo to show that ion-permeation properties are conferred by C-terminal region. We further identify a glutamate residue within a conserved region adjacent to the last two putative TM domains of the protein, that when mutated, affects unitary conductance and ion selectivity, and modulates pore block. We propose that this amino acid is either in the pore or closely associates with the pore. Our results describe important structural motifs of this channel family and lay the groundwork for a mechanistic understanding of how Piezos are mechanically gated and conduct ions. Piezo ion channels function as mechanotransducers involved in vascular development and touch sensing, but their structural features remain unknown. Here the authors find that the C-terminal region of Piezo protein encompasses the pore and identify a glutamate residue within this region involved in ion conduction properties.
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默克尔细胞转导并编码触觉刺激以驱动Aβ型冲动。
影响因子：
64.5
作者：
Ikeda R;Cha M;Ling J;Jia Z;Coyle D;Gu JG
通讯作者：
Gu JG
影响因子：
16.2
作者：
Schmidt, Manuela;Dubin, Adrienne E.;Petrus, Matt J.;Earley, Taryn J.;Patapoutian, Ardem
通讯作者：
Patapoutian, Ardem
DOI：
10.1073/pnas.1221400110
发表时间：
2013-03-19
影响因子：
11.1
作者：
Coste, Bertrand;Houge, Gunnar;Patapoutian, Ardem
通讯作者：
Patapoutian, Ardem
影响因子：
4.8
作者：
Hansen, Scott B.;Wang, Hai-Long;Sine, Steven M.
通讯作者：
Sine, Steven M.
影响因子：
64.8
作者：
Ranade, Sanjeev S.;Woo, Seung-Hyun;Dubin, Adrienne E.;Moshourab, Rabih A.;Wetzel, Christiane;Petrus, Matt;Mathur, Jayanti;Begay, Valerie;Coste, Bertrand;Mainquist, James;Wilson, A. J.;Francisco, Allain G.;Reddy, Kritika;Qiu, Zhaozhu;Wood, John N.;Lewin, Gary R.;Patapoutian, Ardem
通讯作者：
Patapoutian, Ardem