Oligodendrocytes but not astrocytes express apolipoprotein E after injury of rat optic nerve
Oligodendrocytes but not astrocytes express apolipoprotein E after injury of rat optic nerve
复制标题
大鼠视神经损伤后少突胶质细胞表达载脂蛋白E，但星形胶质细胞不表达
作者：
G. Stoll;H. Mueller;B. Trapp;J. Griffin
载脂蛋白 E (apoE) 是一种参与磷脂和胆固醇转运和代谢的脂质结合糖蛋白，在成熟大鼠外周和中枢神经横断后合成和分泌速度加快。在周围神经系统 (PNS) 中，浸润巨噬细胞在华勒变性期间表达 apoE。视神经 (ON) 损伤后，apoE 合成受到显着刺激，但迄今为止尚未鉴定出表达 apoE 的细胞。本研究使用 1 μm 的薄冰冻切片来鉴定横切 ON 中 apoE 的细胞来源。使用 apoE 特异性抗血清和识别不同细胞类型的抗体，通过亲和素-生物素-过氧化物酶复合物免疫细胞化学对连续 1 μm 冷冻切片进行染色：星形胶质细胞的胶质纤维酸性蛋白 (GFAP)、少突胶质细胞的 2',3'-环核苷酸 -3' 磷酸二酯酶 (CNP) 和巨噬细胞的 ED1。在正常 ON 中，星形胶质细胞和少突胶质细胞均为 apoE 阳性。 ON 横断一周后，少突胶质细胞占 apoE 阳性细胞的大多数，而 apoE 免疫反应性从星形胶质细胞体和突起中消失。与 PNS 相比，横断后 7 天 ON 中仅存在少量 ED1/apoE 阳性巨噬细胞。通过使用免疫金标记的超薄冷冻切片，apoE 可以定位于少突胶质细胞的高尔基体中，表明这些细胞的合成。我们的数据表明，少突胶质细胞衍生的 apoE 蛋白可能参与中枢神经系统损伤后髓磷脂脂质的重新分配。
Apolipoprotein E (apoE), a lipid‐binding glycoprotein involved in transport and metabolism of phospholipids and cholesterol, is synthesized and secreted at elevated rates following transection of mature rat peripheral and central nerves. In the peripheral nervous system (PNS) infiltrating macrophages express apoE during Wallerian degeneration. Following injury of the optic nerve (ON) apoE synthesis is significantly stimulated but the apoE‐expressing cells have thus far not been identified. This study used 1 μm and thin cryosections to identify the cellular source of apoE in transected ON. Serial 1 μm frozen sections were stained by avidin‐biotin‐peroxidase complex immunocytochemistry by using a specific antiserum to apoE and by antibodies that identify different cell types: glial fibrillary acidic protein (GFAP) for astrocytes, 2′,3′‐cyclic nucleotide‐3′ phosphodiesterase (CNP) for oligodendrocytes, and ED1 for macrophages. In normal ON both astrocytes and oligodendrocytes were apoE‐positive. One week after ON transection oligodendrocytes accounted for the majority of apoE‐positive cells, while apoE immunoreactivity had disappeared from astroglial cell bodies and processes. In contrast to the PNS only a few ED1/apoE‐positive macrophages were present in ON 7 days after transection. By using immunogold‐labeled ultrathin crysections apoE could be localized in the Golgi apparatus of oligodendrocytes, indicating synthesis by these cells. Our data suggest that oligodendrocyte‐derived apoE protein may participate in the redistribution of myelin lipids after CNS injury.
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神经损伤后，去神经鞘细胞会分泌一种新的蛋白质。
DOI：
10.1073/pnas.80.13.4169
发表时间：
1983
影响因子：
11.1
作者：
Skene,JH;Shooter,EM
通讯作者：
Shooter,EM
DOI：
10.1073/pnas.83.4.1125
发表时间：
1986-02-01
影响因子：
11.1
作者：
IGNATIUS, MJ;GEBICKEHARTER, PJ;SHOOTER, EM
通讯作者：
SHOOTER, EM
影响因子：
56.9
作者：
IGNATIUS, MJ;SHOOTER, EM;MAHLEY, RW
通讯作者：
MAHLEY, RW
DOI：
10.1073/pnas.83.4.1130
发表时间：
1986-02
影响因子：
11.1
作者：
G. Snipes;C. McGuire;J. Norden;J. Freeman
通讯作者：
G. Snipes;C. McGuire;J. Norden;J. Freeman