Effect of ionizing radiation on transgenerational appearance of pun reversions in mice
Effect of ionizing radiation on transgenerational appearance of pun reversions in mice
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电离辐射对小鼠双关语逆转跨代表现的影响
DOI：
10.1093/carcin/20.12.2351
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发表时间：
1999
期刊：
影响因子：
4.7
通讯作者：
R. Schiestl
中科院分区：
文献类型：
--
作者：
N. Carls;R. Schiestl
恶性肿瘤细胞的发展需要多种遗传变化，许多环境诱发的癌症显示出在接触后20年以上的延迟发病。事实上，癌细胞中基因变化的频率高于随机突变所能解释的频率。细胞亚群中的高水平遗传不稳定性可能是由通过许多细胞分裂传递的增变子表型引起的。我们已经确定了辐射对亲代雄性小鼠后代中pun基因座有丝分裂发生的DNA缺失事件的频率和特征的影响。小鼠胚胎中pun标记的逆转是由于70 kb的DNA缺失导致后代出现皮毛斑点。我们发现，照射雄性小鼠导致后代出现大斑点的频率明显较高，这表明在胚胎发育早期诱导了DNA缺失。然而，这些缺失事件发生在照射后的许多细胞分裂中。目前的数据表明，生殖细胞暴露于电离辐射的结果在后代小鼠中诱导延迟的DNA缺失。
Multiple genetic changes are required for the development of a malignant tumor cell and many environmentally induced cancers show a delayed onset of > 20 years following exposure. In fact, the frequency of genetic changes in cancer cells is higher than can be explained by random mutation. A high level of genetic instability in a subpopulation of cells may be caused by a mutator phenotype transmitted through many cell divisions. We have determined the effects of irradiation of parental male mice on the frequency and characteristics of mitotically occurring DNA deletion events at the p un locus in the offspring. Reversion of the p un marker in mouse embryos is due to deletion of 70 kb of DNA resulting in fur spots in the offspring. We found that irradiation of male mice caused a significantly higher frequency of large spots in the offspring, indicative of the induction of DNA deletions early in embryo development. These deletion events occurred, however, many cell divisions after irradiation. The present data indicate that exposure of the germline to ionizing radiation results in induction of delayed DNA deletions in offspring mice.
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低剂量 X 射线诱导的小鼠红眼不稳定突变的逆转。
DOI：
10.1126/science.7985029
发表时间：
1994
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Schiestl,RH;Khogali,F;Carls,N
通讯作者：
Carls,N
DNA 链断裂剂对染色体不稳定性的差异诱导。
DOI：
--
发表时间：
1997
期刊：
Cancer research.
影响因子：
--
作者：
Limoli,CL;Kaplan,MI;Phillips,JW;Adair,GM;Morgan,WF
通讯作者：
Morgan,WF
多氯联苯和 2,3,7,8-四氯二苯并-对二恶英可在体外和体内诱导染色体内重组。
影响因子：
11.2
作者：
Schiestl,RH;Aubrecht,J;Yap,WY;Kandikonda,S;Sidhom,S
通讯作者：
Sidhom,S
影响因子：
11.2
作者：
L. Loeb
通讯作者：
L. Loeb
影响因子：
3.4
作者：
Little, JB;Nagasawa, H;Vetrovs, H
通讯作者：
Vetrovs, H